RGD Reference Report - RalB mobilizes the exocyst to drive cell migration. - Rat Genome Database

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RalB mobilizes the exocyst to drive cell migration.

Authors: Rosse, C  Hatzoglou, A  Parrini, MC  White, MA  Chavrier, P  Camonis, J 
Citation: Rosse C, etal., Mol Cell Biol. 2006 Jan;26(2):727-34.
RGD ID: 8554641
Pubmed: PMID:16382162   (View Abstract at PubMed)
PMCID: PMC1346891   (View Article at PubMed Central)
DOI: DOI:10.1128/MCB.26.2.727-734.2006   (Journal Full-text)

The Ras family GTPases RalA and RalB have been defined as central components of the regulatory machinery supporting tumor initiation and progression. Although it is known that Ral proteins mediate oncogenic Ras signaling and physically and functionally interact with vesicle trafficking machinery, their mechanistic contribution to oncogenic transformation is unknown. Here, we have directly evaluated the relative contribution of Ral proteins and Ral effector pathways to cell motility and directional migration. Through loss-of-function analysis, we find that RalA is not limiting for cell migration in normal mammalian epithelial cells. In contrast, RalB and the Sec6/8 complex or exocyst, an immediate downstream Ral effector complex, are required for vectorial cell motility. RalB expression is required for promoting both exocyst assembly and localization to the leading edge of moving cells. We propose that RalB regulation of exocyst function is required for the coordinated delivery of secretory vesicles to the sites of dynamic plasma membrane expansion that specify directional movement.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
regulation of exocyst assembly involved_inIDA 8554641PMID:16382162UniProt 
regulation of exocyst localization involved_inIDA 8554641PMID:16382162UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Ralb  (RAS like proto-oncogene B)

Additional Information