RGD Reference Report - Caveolae: biochemical analysis. - Rat Genome Database

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Caveolae: biochemical analysis.

Authors: Chatenay-Rivauday, C  Cakar, ZP  Jeno, P  Kuzmenko, ES  Fiedler, K 
Citation: Chatenay-Rivauday C, etal., Mol Biol Rep. 2004 Jun;31(2):67-84.
RGD ID: 8554261
Pubmed: PMID:15293782   (View Abstract at PubMed)

Caveolae appear in a multitude of processes encompassing growth regulation and trafficking. We demonstrate the abundant presence of ESA/reggie-1/flotillin-2, ATP synthase beta subunit and annexin V in endothelial caveolae by immunopurification of caveolae from vascular endothelial membrane. Five proteins are abundant in a caveolin-1 protein complex, analyzed by sucrose gradient velocity sedimentation following octyl-beta-D-glucopyranoside extraction. Caveolin-1 alpha interacts with caveolin-1beta, caveolin-2, actin, the microsomal form of NADH cytochrome B5 reductase and ESA/reggie-1/flotillin-2 as shown by co-immunoprecipitation. We propose the concept that ATP biosynthesis in caveolae regulates mechanosignaling and is induced by membrane depolarization and a proton gradient. Pressure stimuli and metabolic changes may trigger gene regulation in endothelial cells, involving a nuclear conformer of caveolin-1, shown here with an epitope-specific caveolin-1 antibody, and immediate response of ion channel activity, regulated by ESA/reggie-1/flotillin-2.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cav1Ratprotein binding enablesIPIUniProtKB:P41350-1PMID:15293782IntAct 
Cav1Ratprotein binding enablesIPIUniProtKB:P41350-2PMID:15293782IntAct 

Objects Annotated

Genes (Rattus norvegicus)
Cav1  (caveolin 1)


Additional Information