RGD Reference Report - CaM kinase II-dependent phosphorylation of myogenin contributes to activity-dependent suppression of nAChR gene expression in developing rat myotubes. - Rat Genome Database

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CaM kinase II-dependent phosphorylation of myogenin contributes to activity-dependent suppression of nAChR gene expression in developing rat myotubes.

Authors: Tang, H  Macpherson, P  Argetsinger, LS  Cieslak, D  Suhr, ST  Carter-Su, C  Goldman, D 
Citation: Tang H, etal., Cell Signal. 2004 May;16(5):551-63.
RGD ID: 8553747
Pubmed: PMID:14751541   (View Abstract at PubMed)

During development of the neuromuscular junction (NMJ), extrajunctional expression of genes, whose products are destined for the synapse, is suppressed by muscle activity. One of the best-studied examples of activity-dependent gene regulation in muscle are those encoding nicotinic acetylcholine receptor (nAChR) subunits. We recently showed that nAChR gene expression is inhibited by calcium/calmodulin-dependent protein kinase II (CaMKII) and CaMKII inhibitors block activity-dependent suppression of these genes. Here we report results investigating the mechanism by which CaMKII suppresses nAChR gene expression. We show that the muscle helix-loop-helix transcription factor, myogenin, is necessary for activity-dependent control of nAChR gene expression in cultured rat myotubes and is a substrate for CaMKII both in vitro and in vivo. CaMKII phosphorylation of myogenin is induced by muscle activity and this phosphorylation influences DNA binding and transactivation. Thus we have identified a signaling mechanism by which muscle activity controls nAChR gene expression in developing muscle.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MyogRatpositive regulation of transcription by RNA polymerase II involved_inIDA PMID:14751541UniProt 
MyogRatresponse to muscle activity involved in regulation of muscle adaptation involved_inIDA PMID:14751541UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MyogRatnucleus located_inIDA PMID:14751541UniProt 
MyogRatprotein-DNA complex part_ofIDA PMID:14751541UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MyogRatRNA polymerase II cis-regulatory region sequence-specific DNA binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Myog  (myogenin)


Additional Information