RGD Reference Report - An NGF-responsive element targets myo-inositol monophosphatase-1 mRNA to sympathetic neuron axons. - Rat Genome Database

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An NGF-responsive element targets myo-inositol monophosphatase-1 mRNA to sympathetic neuron axons.

Authors: Andreassi, C  Zimmermann, C  Mitter, R  Fusco, S  De Vita, S  Saiardi, A  Riccio, A 
Citation: Andreassi C, etal., Nat Neurosci. 2010 Mar;13(3):291-301. doi: 10.1038/nn.2486. Epub 2010 Jan 31.
RGD ID: 8553528
Pubmed: PMID:20118926   (View Abstract at PubMed)
DOI: DOI:10.1038/nn.2486   (Journal Full-text)

mRNA localization is an evolutionary conserved mechanism that underlies the establishment of cellular polarity and specialized cell functions. To identify mRNAs localized in subcellular compartments of developing neurons, we took an original approach that combines compartmentalized cultures of rat sympathetic neurons and sequential analysis of gene expression (SAGE). Unexpectedly, the most abundant transcript in axons was mRNA for myo-inositol monophosphatase-1 (Impa1), a key enzyme that regulates the inositol cycle and the main target of lithium in neurons. A novel localization element within the 3' untranslated region of Impa1 mRNA specifically targeted Impa1 transcript to sympathetic neuron axons and regulated local IMPA1 translation in response to nerve growth factor (NGF). Selective silencing of IMPA1 synthesis in axons decreased nuclear CREB activation and induced axonal degeneration. These results provide insights into mRNA transport in axons and reveal a new NGF-responsive localization element that directs the targeting and local translation of an axonal transcript.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Impa1Rataxon located_inIDA PMID:20118926BHF-UCL 
NefmRataxon located_inIDA PMID:20118926BHF-UCL 
Impa1Ratneuronal cell body located_inIDA PMID:20118926BHF-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Impa1  (inositol monophosphatase 1)
Nefm  (neurofilament medium chain)


Additional Information