RGD Reference Report - A factor derived from polymorphonuclear leukocytes enhances interleukin-1-induced synovial cell collagenase and prostaglandin E2 production in rats. - Rat Genome Database

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A factor derived from polymorphonuclear leukocytes enhances interleukin-1-induced synovial cell collagenase and prostaglandin E2 production in rats.

Authors: Hashida, R  Kuwada, M  Chiba, KI  Horizoe, T  Shirota, H  Nagai, Y 
Citation: Hashida R, etal., Eur J Biochem. 1996 Mar 1;236(2):517-22.
RGD ID: 7800677
Pubmed: PMID:8612624   (View Abstract at PubMed)

We found that short-term culture medium and homogenate of casein-induced rat peritoneal polymorphonuclear leukocytes (PMN) markedly induced collagenase and prostaglandin E2 (PGE2) production by normal rat synovial cells and these effects were abrogated by anti-(rat interleukin-1 alpha) (IL-1 alpha) polyclonal antibodies. However, collagenase activity and PGE2 induced by recombinant rat IL-1 alpha were less than those induced by rat PMN culture medium. It was also proved by radioimmunoassay that rat PMN culture medium contains a relatively small amount of IL-1 alpha. The introduction of IL-1 alpha-deleted PMN culture medium and recombinant rat IL-1 alpha together into the synovial cell culture system revealed that IL-1 alpha deleted PMN culture medium has a significant enhancing activity on IL-1 alpha-induced synovial cell collagenase and PGE2 production. This new factor, which was shown to be a negatively charged protein of about 80 kDa, may have important roles in connective tissue destruction and chronic inflammation in diseases such as rheumatoid arthritis.




Biological Process

  
Object Symbol
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Term
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Evidence
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Original Reference(s)
Il1aRatpositive regulation of prostaglandin secretion  IDA  RGD 


Genes (Rattus norvegicus)
Il1a  (interleukin 1 alpha)