RGD Reference Report - Influence of sleep deprivation on expression of MKK4 and c-fos in the mandibular condylar cartilage of rats. - Rat Genome Database

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Influence of sleep deprivation on expression of MKK4 and c-fos in the mandibular condylar cartilage of rats.

Authors: Chen, J  Wu, G  Zhu, G  Wang, P  Chen, H  Zhao, H 
Citation: Chen J, etal., Br J Oral Maxillofac Surg. 2013 Dec;51(8):e250-5. doi: 10.1016/j.bjoms.2013.06.010. Epub 2013 Jul 13.
RGD ID: 7495827
Pubmed: PMID:23859770   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bjoms.2013.06.010   (Journal Full-text)

The aim of this study was to investigate the changes in expression of mitogen-activated protein kinase kinase 4 (MKK4) and c-fos in the mandibular condylar cartilage of rats that had been subjected to sleep deprivation. One hundred and twenty female Wistar rats were randomly divided into 6 groups with 20 in each: sleep deprivation for 2 days, 4 days, 6 days, and 8 days, large-platform controls, and cage controls. After sleep deprivation by the modified multiple platform method the sleep-deprived rats were killed. The large-platform and cage control rats were killed at the same time as the rats deprived of sleep for 8 days. Haematoxylin and eosin were used to record the morphological changes in cartilage, and immunohistochemistry and real-time quantitative polymerase chain reaction (PCR) were used to detect the expression of MKK4 and c-fos. Pathological alterations were apparent after 6 and 8 days of sleep deprivation. Compared with control groups, the expression of MKK4 in the sleep-deprived groups was lower, while that of c-fos was higher. As the duration of sleep deprivation increased, the expression of MKK4 decreased. These results indicate that the variation in expression of MKK4 and c-fos may be correlated with pathological changes induced by sleep deprivation in mandibular condylar cartilage in rats.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Sleep Deprivation  ISOMap2k4 (Rattus norvegicus)7495827; 7495827mRNA and protein:decreased expression:mandible condylar processRGD 
Sleep Deprivation  IEP 7495827mRNA and protein:decreased expression:mandible condylar processRGD 

Objects Annotated

Genes (Rattus norvegicus)
Map2k4  (mitogen activated protein kinase kinase 4)

Genes (Mus musculus)
Map2k4  (mitogen-activated protein kinase kinase 4)

Genes (Homo sapiens)
MAP2K4  (mitogen-activated protein kinase kinase 4)


Additional Information