RGD Reference Report - Rab8b and its interacting partner TRIP8b are involved in regulated secretion in AtT20 cells. - Rat Genome Database

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Rab8b and its interacting partner TRIP8b are involved in regulated secretion in AtT20 cells.

Authors: Chen, S  Liang, MC  Chia, JN  Ngsee, JK  Ting, AE 
Citation: Chen S, etal., J Biol Chem 2001 Apr 20;276(16):13209-16. Epub 2001 Jan 24.
RGD ID: 737646
Pubmed: PMID:11278749   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M010798200   (Journal Full-text)

Rab proteins are a family of small GTPases that regulate intracellular vesicle traffic. Rab8b, because of its homology with Rab8, has been suggested to function in vesicle transport to the plasma membrane. Using the yeast two-hybrid system, we identified a Rab8b interacting clone, termed TRIP8b, from a rat brain cDNA library. The gene encodes a 66-kDa protein with homology to the peroxisomal targeting signal 1 receptor. The interaction between Rab8b and TRIP8b was further verified by in vitro binding assays and co-immunoprecipitation studies. Additional experiments with Rab8b mutants demonstrated that Rab8b requires a guanine nucleotide but not prenylation for its interaction with TRIP8b. Western immunoblot analysis showed that TRIP8b was primarily expressed in brain. Subcellular fractionation of AtT20 cells revealed that TRIP8b was present in both cytosolic and membrane fractions. To investigate the function of Rab8b and TRIP8b in secretion, we examined the release of ACTH from AtT20 cells. Results from stable cell lines expressing Rab8b or TRIP8b indicated that both proteins had a stimulatory effect on cAMP-induced secretion of ACTH. In summary, these data suggest that Rab8b and TRIP8b interact with each other and are involved in the regulated secretory pathway in AtT20 cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rab8bRatpositive regulation of cell projection organization  IDA  RGD 
Pex5lRatpositive regulation of corticotropin secretion  IDA  RGD 
Rab8bRatpositive regulation of corticotropin secretion  IDA  RGD 
Pex5lRatregulated exocytosis  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Pex5lRatcell tip  IDA  RGD 
Rab8bRatcell tip  IDA  RGD 
Pex5lRatperinuclear region of cytoplasm  IDA  RGD 
Rab8bRatperinuclear region of cytoplasm  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Pex5lRatsmall GTPase binding  IPIRab8b (Rattus norvegicus) RGD 
Rab8bRatTPR domain binding  IPIPex5l (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pex5l  (peroxisomal biogenesis factor 5-like)
Rab8b  (RAB8B, member RAS oncogene family)


Additional Information