RGD Reference Report - Cloning of a cDNA encoding a non-isopeptide-selective subtype of the endothelin receptor. - Rat Genome Database

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Cloning of a cDNA encoding a non-isopeptide-selective subtype of the endothelin receptor.

Authors: Sakurai, T  Yanagisawa, M  Takuwa, Y  Miyazaki, H  Kimura, S  Goto, K  Masaki, T 
Citation: Sakurai T, etal., Nature 1990 Dec 20-27;348(6303):732-5.
RGD ID: 730808
Pubmed: PMID:2175397   (View Abstract at PubMed)
DOI: DOI:10.1038/348732a0   (Journal Full-text)

Endothelin-1 was initially identified as a 21-residue potent vasoconstrictor peptide produced by vascular endothelial cells, but was subsequently found to have many effects on both vascular and non-vascular tissues. The discovery of three isopeptides of the endothelin family, ET-1, ET-2 and ET-3, each possessing a diverse set of pharmacological activities of different potency, suggested the existence of several different endothelin receptor subtypes. Endothelins may elicit biological responses by various signal-transduction mechanisms, including the G protein-coupled activation of phospholipase C and the activation of voltage-dependent Ca2+ channels. Thus, different subtypes of the endothelin receptor may use different signal-transduction mechanisms. Here we report the cloning of a complementary DNA encoding one subtype belonging to the superfamily of G protein-coupled receptors. COS-7 cells transfected with the cDNA express specific and high-affinity binding sites for endothelins, responding to binding by the production of inositol phosphates and a transient increase in the concentration of intracellular free Ca2+. The three endothelin isopeptides are roughly equipotent in displacing 125I-labelled ET-1 binding and causing Ca2+ mobilization. A messenger RNA corresponding to the cDNA is detected in many rat tissues including the brain, kidney and lung but not in vascular smooth muscle cells. These results indicate that this cDNA encodes a 'nonselective' subtype of the receptor which is different from the vascular smooth muscle receptor.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
EdnrbRatpositive regulation of cytosolic calcium ion concentration  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
EdnrbRatendothelin receptor activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ednrb  (endothelin receptor type B)


Additional Information