RGD Reference Report - Role of NHE isoforms in mediating bicarbonate reabsorption along the nephron. - Rat Genome Database

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Role of NHE isoforms in mediating bicarbonate reabsorption along the nephron.

Authors: Wang, T  Hropot, M  Aronson, PS  Giebisch, G 
Citation: Wang T, etal., Am J Physiol Renal Physiol 2001 Dec;281(6):F1117-22.
RGD ID: 730257
Pubmed: PMID:11704563   (View Abstract at PubMed)

This study assessed the functional role of Na(+)/H(+) exchanger (NHE) isoforms NHE3 and NHE2 in the proximal tubule, loop of Henle, and distal convoluted tubule of the rat kidney by comparing sensitivity of transport to inhibition by Hoe-694 (an agent known to inhibit NHE2 but not NHE3) and S-3226 (an agent with much higher affinity for NHE3 than NHE2). Rates of transport of fluid (J(v)) and HCO(3)(-) (J(HCO3)) were studied by in situ microperfusion. In the proximal tubule, addition of ethylisopropylamiloride or S-3226 significantly reduced J(v) and J(HCO3), but addition of Hoe-694 caused no significant inhibition. In the loop of Henle, J(HCO3) was also inhibited by S-3226 and not by Hoe-694, although much higher concentrations of S-3226 were required than what was necessary to inhibit transport in the proximal tubule. In contrast, in the distal convoluted tubule, J(HCO3) was inhibited by Hoe-694 but not by S-3226. These results are consistent with the conclusion that NHE2 rather than NHE3 is the predominant isoform responsible for apical membrane Na(+)/H(+) exchange in the distal convoluted tubule, whereas NHE3 is the predominant apical isoform in the proximal tubule and possibly also in the loop of Henle.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc9a2Ratapical plasma membrane  TAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc9a2  (solute carrier family 9 member A2)

Objects referenced in this article
Gene Slc9a3 solute carrier family 9 member A3 Rattus norvegicus

Additional Information