RGD Reference Report - mu opiate receptor: cDNA cloning and expression. - Rat Genome Database

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mu opiate receptor: cDNA cloning and expression.

Authors: Wang, JB  Imai, Y  Eppler, CM  Gregor, P  Spivak, CE  Uhl, GR 
Citation: Wang JB, etal., Proc Natl Acad Sci U S A 1993 Nov 1;90(21):10230-4.
RGD ID: 729033
Pubmed: PMID:8234282   (View Abstract at PubMed)
PMCID: PMC47748   (View Article at PubMed Central)

mu opiate receptors recognize morphine with high affinity. A 2.1-kb rat brain cDNA whose predicted translation product displays 63% identity with recently described delta and kappa opiate receptor sequences was identified through polymerase chain reaction and cDNA homology approaches. This cDNA recognizes a 10.5-kb mRNA that is expressed in thalamic neurons. COS-cell expression confers naloxonazine-, Na(+)-, and GTP-sensitive binding of mu but not delta or kappa opioid ligands. Expressing cells bind morphine, [D-Ala2,N-methyl-Phe4,glyol5]enkephalin (DAMGO), and [D-Ala2,D-Leu5]enkephalin (DADLE) with nanomolar or subnanomolar affinities, defining a mu opiate receptor that avidly recognizes analgesic and euphoric opiate drugs and opioid peptides.



Objects referenced in this article
Gene Oprm1 opioid receptor, mu 1 Rattus norvegicus

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