RGD Reference Report - Functional expression of corticotropin-releasing hormone (CRH) receptor 1 in cultured rat microglia. - Rat Genome Database

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Functional expression of corticotropin-releasing hormone (CRH) receptor 1 in cultured rat microglia.

Authors: Wang, W  Ji, P  Riopelle, RJ  Dow, KE 
Citation: Wang W, etal., J Neurochem 2002 Jan;80(2):287-94.
RGD ID: 727696
Pubmed: PMID:11902119   (View Abstract at PubMed)

Corticotropin-releasing hormone (CRH), known as a key regulator of the hypothalamic-pituitary-adrenal axis response to stress, elicits its biological effects by binding to two membrane receptors (CRH-R1 and CRH-R2). The present studies examined the presence of functional expression of CRH receptors in cultured microglia of rat. CRH-R1 mRNA and protein were detected by reverse transcriptase polymerase chain reaction (RT-PCR), western blotting and receptor chemical cross-linking assay in cultured microglia. CRH-R2 mRNA was undetectable by RT-PCR. The radioligand binding analysis using [125I]Tyr-rat/human CRH revealed a high affinity binding site (Kd of 1.2 nm and Bmax of 84 fmol/mg of protein). Competition studies using CRH and related peptides indicated kinetic and pharmacological characteristics consistent with the CRH-R1 receptor subtype. Receptor chemical cross-linking assay demonstrated a single band of CRH receptor with a molecular weight of -77 kDa, which was inhibited in the presence of excess unlabeled rat/human CRH in a dose-dependent manner and inhibited by a CRH receptor antagonist astressin. Functional coupled cAMP production in cultured microglia was stimulated by exogenous addition of CRH and related peptides in a dose-dependent manner and blocked by astressin. Our findings suggest the functional expression of CRH-R1 receptor in rat microglia, indicating an important mechanism of interaction between immune and neuroendocrine systems in brain physiological and pathological conditions.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Crhr1RatG protein-coupled receptor activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Crhr1  (corticotropin releasing hormone receptor 1)


Additional Information