RGD Reference Report - Identification of a new outwardly rectifying Cl- channel that belongs to a subfamily of the ClC Cl- channels. - Rat Genome Database

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Identification of a new outwardly rectifying Cl- channel that belongs to a subfamily of the ClC Cl- channels.

Authors: Sakamoto, H  Kawasaki, M  Uchida, S  Sasaki, S  Marumo, F 
Citation: Sakamoto H, etal., J Biol Chem 1996 Apr 26;271(17):10210-6.
RGD ID: 727659
Pubmed: PMID:8626585   (View Abstract at PubMed)

A new outwardly rectifying Cl- channels (ORCC) that belongs to ClC Cl- channel family has been identified from rat kidney and designated as ClC-5. ClC-5 cDNA encodes a polypeptide of 746 amino acids, which is indicated by hydrophobicity analysis to have structural features that are common of the ClC family. However, the amino acid sequence was weakly homologous to those of other ClC Cl- channels except for ClC-3, which we recently identified as a Ca2+-sensitive ORCC. Northern blot analysis of rat tissues showed that ClC-5 mRNA was predominantly expressed in the kidney and colon. To characterize the functional properties of ClC-5 by whole cell patch-clamp technique, we established the stably transfected CHO-K1 cell line using intranuclear microinjection technique. The transfected cells induced outwardly rectifying and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid-sensitive Cl- currents on whole cell configuration. Following the identification of two highly homologous ORCCs, ClC-3 and ClC-5, a new subfamily encoding ORCC has emerged in the ClC family. Furthermore, ClC-5 was almost identical to a partial sequence of human cDNA that is related to Dent's disease. The molecular structure and functional properties of ClC-5 will provide an important insight into ORCCs and the pathogenesis of Dent's disease.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Clcn5Ratchloride transport  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Clcn5Ratvoltage-gated chloride channel activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Clcn5  (chloride voltage-gated channel 5)


Additional Information