RGD Reference Report - RNA interference-mediated silencing of synaptotagmin IX, but not synaptotagmin I, inhibits dense-core vesicle exocytosis in PC12 cells. - Rat Genome Database

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RNA interference-mediated silencing of synaptotagmin IX, but not synaptotagmin I, inhibits dense-core vesicle exocytosis in PC12 cells.

Authors: Fukuda, M 
Citation: Fukuda M Biochem J. 2004 Jun 15;380(Pt 3):875-9.
RGD ID: 7241274
Pubmed: PMID:15015935   (View Abstract at PubMed)
PMCID: PMC1224215   (View Article at PubMed Central)
DOI: DOI:10.1042/BJ20040096   (Journal Full-text)

Although PC12 cells express three synaptotagmin isoforms (Syts I, IV and IX), all of which have been proposed to regulate dense-core vesicle exocytosis, it remains unknown which of the Sytisoforms acts as the major Ca2+ sensor for dense-core vesicle exocytosis. In the present study, it has been shown by immunoaffinity purification and immunocytochemistry that Syts I and IX, but not Syt IV, are present on the same secretory vesicles in PC12 cells. Silencing of Syt IX with specific small interfering RNA significantly reduced high KCl-dependent neuropeptide Y secretion from PC12 cells, whereas silencing of Syt I with specific small interfering RNA had no significant effect. The results indicate that Syts I and IX are not functionally equivalent and that Syt IX, and not Syt I, is indispensable for the regulation of Ca2+-dependent dense-core vesicle exocytosis in PC12 cells.




Biological Process

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Syt9Ratregulation of calcium ion-dependent exocytosis  IMP  RGD 

Cellular Component

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Syt1Ratsecretory granule  IDA  RGD 
Syt9Ratsecretory granule  IDA  RGD 


Genes (Rattus norvegicus)
Syt1  (synaptotagmin 1) Syt9  (synaptotagmin 9)