RGD Reference Report - Formation of cellular projections in neural progenitor cells depends on SK3 channel activity. - Rat Genome Database

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Formation of cellular projections in neural progenitor cells depends on SK3 channel activity.

Authors: Liebau, S  Vaida, B  Proepper, C  Grissmer, S  Storch, A  Boeckers, TM  Dietl, P  Wittekindt, OH 
Citation: Liebau S, etal., J Neurochem. 2007 Jun;101(5):1338-1350.
RGD ID: 7175515
Pubmed: PMID:17459146   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1471-4159.2006.04437.x   (Journal Full-text)

Ion channels are potent modulators for developmental processes in progenitor cells. In a screening approach for different ion channels in neural progenitor cells (NPCs) we observed a 1-ethyl-2-benzimidazolinone (1-EBIO) activated inward current, which could be blocked by scyllatoxin (ScTX, IC50=2+/- 0.3 nmol/L). This initial evidence for the expression of the small conductance Ca2+ activated K+-channel SK3 was confirmed by the detection of SK3 transcripts and protein in NPCs. Interestingly, SK3 proteins were highly expressed in non-differentiated NPCs with a focused localization in lamellipodia as well as filopodial structures. The activation of SK3 channels using 1-EBIO lead to an immediate filopodial sprouting and the translocation of the protein into these novel filopodial protrusions. Both effects could be prevented by the pre-incubation of NPCs with ScTX. Our study gives first evidence that the formation and prolongation of filopodia in NPCs is, at least in part, effectively induced and regulated by SK3 channels.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnn3Ratpotassium ion transport  IMP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnn3Ratfilopodium  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnn3Ratsmall conductance calcium-activated potassium channel activity  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kcnn3  (potassium calcium-activated channel subfamily N member 3)


Additional Information