RGD Reference Report - Pyk2/CAKbeta tyrosine kinase activity-mediated angiogenesis of pulmonary vascular endothelial cells. - Rat Genome Database

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Pyk2/CAKbeta tyrosine kinase activity-mediated angiogenesis of pulmonary vascular endothelial cells.

Authors: Tang, H  Hao, Q  Fitzgerald, T  Sasaki, T  Landon, EJ  Inagami, T 
Citation: Tang H, etal., J Biol Chem 2002 Feb 15;277(7):5441-7.
RGD ID: 70597
Pubmed: PMID:11739395   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M110673200   (Journal Full-text)

Endothelial cell spreading, migration, and morphogenesis are essential for angiogenesis, the formation of new blood vessels. In the present study, we explored roles of tyrosine kinase Pyk2 in angiogenesis of pulmonary endothelial cells. We found that tyrosine kinase Pyk2 was particularly enriched in pulmonary vascular endothelial cells and lung, a major organ site for tumor metastasis. By using adenovirus-mediated expression of various Pyk2 mutants, we demonstrated that Pyk2 tyrosine kinase activity was essential for the pulmonary vascular endothelial cell spreading, migration, morphogenesis, as well as pulmonary vein and artery angiogenesis ex vivo. We further showed that Pyk2 kinase activity was required for the expression of focal adhesion kinase, p130Crk-associated substrate, and its homologue human enhancer of filamentation 1, thus regulating formation of focal adhesions and cytoskeletal reorganization. These results indicate that Pyk2 plays a crucial role in the pulmonary endothelial cell motility such as spreading and migration necessary for angiogenesis.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ptk2bRatangiogenesis  IMP  RGD 
Ptk2bRatblood vessel endothelial cell migration  IMP  RGD 
Ptk2bRatcell adhesion  IMP  RGD 
Ptk2bRatstress fiber assembly  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ptk2b  (protein tyrosine kinase 2 beta)


Additional Information