RGD Reference Report - Pancreastatin, a chromogranin A-derived peptide, activates Galpha(16) and phospholipase C-beta(2) by interacting with specific receptors in rat heart membranes. - Rat Genome Database

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Pancreastatin, a chromogranin A-derived peptide, activates Galpha(16) and phospholipase C-beta(2) by interacting with specific receptors in rat heart membranes.

Authors: Gonzalez-Yanes, C  Santos-Alvarez, J  Sanchez-Margalet, V 
Citation: Gonzalez-Yanes C, etal., Cell Signal. 2001 Jan;13(1):43-9.
RGD ID: 6907050
Pubmed: PMID:11257446   (View Abstract at PubMed)

Pancreastatin (PST) is one of the chromogranin A (CGA)-derived peptides with known biological activity. It has a general inhibitory effect on secretion in many exocrine and endocrine systems including the heart atrium. Besides, a role of PST as a counter-regulatory peptide of insulin action has been proposed in the light of its effects on glucose and lipid metabolism in the liver and adipose tissue, where receptors and signaling have been described. Galpha(q/11) pathway seems to mediate PST action. Since PST has been shown to function as a typical calcium-dependent hormone, and increased plasma levels have been found in essential hypertension correlating with catecholamines, we sought to study its possible interaction and signaling in heart membranes. Here, we are characterizing specific PST binding sites and signaling in rat heart membranes. We have found that PST receptor has a K(d) of 0.5 nM and a B(max) of 34 fmol/mg of protein. The PST binding is inhibited by guanine nucleotides, suggesting the functional coupling of the receptor with GTP binding proteins (G proteins). Moreover, PST dose-dependently increases GTP binding to rat heart membranes. Finally, we have studied PST signaling-effector system by measuring phospholipase C (PLC) activity using blocking antibodies against different G proteins and PLC isoforms. We have found that PST stimulates PLCbeta(2)>PLCbeta(1)>PLCbeta(3) by activating Galpha(16) in rat heart membranes. These data suggest that PST may modulate the cardiac function.




Biological Process

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
ChgaRatpositive regulation of phospholipase C-activating G protein-coupled receptor signaling pathway  IDA  RGD 

RGD Manual Annotations


  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
CHGAHumanG protein mediated signaling pathway  ISOChga (Rattus norvegicus) RGD 
ChgaRatG protein mediated signaling pathway  IDA  RGD 
ChgaMouseG protein mediated signaling pathway  ISOChga (Rattus norvegicus) RGD 

Genes (Rattus norvegicus)
Chga  (chromogranin A)

Genes (Mus musculus)
Chga  (chromogranin A)

Genes (Homo sapiens)
CHGA  (chromogranin A)