RGD Reference Report - Cloning of rat grp75, an hsp70-family member, and its expression in normal and ischemic brain. - Rat Genome Database

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Cloning of rat grp75, an hsp70-family member, and its expression in normal and ischemic brain.

Authors: Massa, SM  Longo, FM  Zuo, J  Wang, S  Chen, J  Sharp, FR 
Citation: Massa SM, etal., J Neurosci Res. 1995 Apr 15;40(6):807-19.
RGD ID: 6784525
Pubmed: PMID:7629893   (View Abstract at PubMed)
DOI: DOI:10.1002/jnr.490400612   (Journal Full-text)

Following metabolic stress a variety of gene products are induced in cells in the brain, some of which may protect the tissue from subsequent stresses. The heat shock proteins (hsps), in particular hsp70, have been widely studied in this context, but evidence for the involvement of known hsps in protection of the CNS is inconclusive. We have therefore undertaken the search for other stress-induced proteins which may mitigate ischemic injury. Beginning with degenerate RT-PCR, we have isolated a rat-brain cDNA encoding a protein highly similar to human grp75, a mitochondrial member of the hsp70-family of stress proteins. It is also highly similar to two non-mitochondrial proteins; mortalin, a senescence-related gene product, and pbp74, a protein implicated in B-cell peptide processing. Sequence structure and phylogenetic analyses predict mitochondrial localization and induction by a calcium ionophore and glucose deprivation in PC12 cells support its identification as rat grp75. In situ analysis of normal brain reveals an unusual distribution, with very high expression in neurons of the basal forebrain, reticular and subthalamic nuclei, globus pallidus, amygdala and elsewhere. grp75-mRNA is upregulated following focal brain ischemia in a distinctive fashion. When the degree of injury is small, induction occurs in the area of injury, similar to the pattern observed for hsp70. However, when the injury is extensive, hsr is upregulated in neurons outside the ischemic area. The induction of grp75 may represent a sensitive marker of metabolically compromised tissue.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HSPA9Humanbrain ischemia  ISOHspa9 (Rattus norvegicus)mRNA:increased expression:brainRGD 
Hspa9Ratbrain ischemia  IEP mRNA:increased expression:brainRGD 
Hspa9Mousebrain ischemia  ISOHspa9 (Rattus norvegicus)mRNA:increased expression:brainRGD 

Objects Annotated

Genes (Rattus norvegicus)
Hspa9  (heat shock protein family A (Hsp70) member 9)

Genes (Mus musculus)
Hspa9  (heat shock protein 9)

Genes (Homo sapiens)
HSPA9  (heat shock protein family A (Hsp70) member 9)


Additional Information