RGD Reference Report - Complete amino acid sequence of rat L-type pyruvate kinase deduced from the cDNA sequence. - Rat Genome Database

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Complete amino acid sequence of rat L-type pyruvate kinase deduced from the cDNA sequence.

Authors: Inoue, H  Noguchi, T  Tanaka, T 
Citation: Inoue H, etal., Eur J Biochem 1986 Jan 15;154(2):465-9.
RGD ID: 633541
Pubmed: PMID:3002799   (View Abstract at PubMed)

cDNA clones, containing the entire coding region of rat L-type pyruvate kinase, were isolated and their nucleotide sequences were determined by the dideoxy-chain-termination method. The predicted coding region, which spans 543 amino acids, established the complete amino acid sequence of the L-type isozyme of pyruvate kinase for the first time. The deduced amino acid sequence of the L type has one phosphorylation site in its amino terminus and shows about 68% and 48% homologies with M1-type pyruvate kinase of chicken and yeast pyruvate kinase respectively. Domain A exhibits higher homology than domains B and C. The residues in the active site of the L-type enzyme of rats, lying between domains B and A2, are rather different from those of the M1-type enzyme of chickens, but other residues constituting the active site are identical with those of the chicken M1 type except for one amino acid substitution.



Gene Pklr pyruvate kinase L/R Rattus norvegicus