RGD Reference Report - Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B. - Rat Genome Database

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Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B.

Authors: Mann, SS  Hammarback, JA 
Citation: Mann SS and Hammarback JA, J Biol Chem 1994 Apr 15;269(15):11492-7.
RGD ID: 633201
Pubmed: PMID:7908909   (View Abstract at PubMed)

Light chain 3 (LC3) is a subunit of the neuronal microtubule-associated proteins (MAPs), MAP1A and MAP1B. Recent findings show that the cDNAs for MAP1A and MAP1B encode polyproteins that contain the MAP1A or MAP1B heavy chain and a LC2 or LC1 subunit, respectively. We have sequenced a cDNA encoding rat LC3, and its sequence is not found in the MAP1A/LC2 or MAP1B/LC1 polyprotein cDNAs. The deduced amino acid sequence of LC3 is highly conserved between rat and mouse. Rat LC3 is a 16.4-kDa protein with a predicted pI of 9.2. It is encoded on a 1.7-kilobase mRNA. Our anti-LC3 antiserum shows that LC3 is abundant only in neurons and that the majority of LC3 in brain co-purifies with microtubules. Purified recombinant rat LC3 retains the ability to associate with microtubules assembled in the presence of brain MAPs and with microtubules assembled from purified tubulin. We propose that LC3 functions primarily as a MAP1A and MAP1B subunit and that its expression could regulate the microtubule binding activity of MAP1A and MAP1B.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Map1lc3bRatpositive regulation of protein binding  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Map1bRataxon  IDA  RGD 
Map1lc3bRataxon  IDA  RGD 
Map1bRatdendrite  IDA  RGD 
Map1lc3bRatdendrite  IDA  RGD 
Map1aRatmicrotubule associated complex  IDA  RGD 
Map1bRatmicrotubule associated complex  IDA  RGD 
Map1lc3bRatmicrotubule associated complex  IDA  RGD 
Map1bRatneuronal cell body  IDA  RGD 
Map1lc3bRatneuronal cell body  IDA  RGD 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Map1a  (microtubule-associated protein 1A)
Map1b  (microtubule-associated protein 1B)
Map1lc3b  (microtubule-associated protein 1 light chain 3 beta)


Additional Information