RGD Reference Report - Primary structure and androgen regulation of a 20-kilodalton protein specific to rat ventral prostate. - Rat Genome Database

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Primary structure and androgen regulation of a 20-kilodalton protein specific to rat ventral prostate.

Authors: Ho, KC  Snoek, R  Quarmby, V  Viskochil, DH  Rennie, PS  Wilson, EM  French, FS  Bruchovsky, N 
Citation: Ho KC, etal., Biochemistry 1989 Jul 25;28(15):6367-73.
RGD ID: 632205
Pubmed: PMID:2477055   (View Abstract at PubMed)

Nuclear and cytosolic forms of a 20-kdalton rat ventral prostate protein were purified and partially sequenced from their N-termini. Isolated nuclei were treated with micrococcal nuclease and extracted in 0.6 M NaCl, and proteins were separated by affinity chromatography on Matrex gel green A, ammonium sulfate fractionation, and fast protein liquid chromatography on Superose 12. The 43 amino acid N-terminal sequence of the nuclear 20-kdalton protein was identical with the cytosolic protein except it lacked 7 N-terminal amino acids present in the cytosolic form. The DNA sequence of a full-length complementary DNA clone isolated from a ventral prostate gt11 library extended the N-terminal sequence of the cytosolic form by an additional nine amino acids from the predicted initiation methionine. The cDNA included the nucleotide sequence for the 43 amino acid N-terminal sequence of the purified 20-kdalton protein and predicted molecular weights of 16,686, 17,521, and 18,650, respectively, for the nuclear, cytoplasmic, and nonprocessed proteins. Northern blot analyses of reproductive tract tissue RNAs using the 20-kdalton protein cDNA as probe revealed a single mRNA species of 0.92 kb detectable only in extracts of rat ventral prostate. Expression of the 0.92-kb mRNA was androgen dependent since the mRNA was undetectable in extracts obtained 4 days after castration and was restored 16 h after restimulation with androgen.

Objects referenced in this article
Gene Andpro androgen regulated protein Rattus norvegicus

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