RGD Reference Report - TRPC6 is a candidate channel involved in receptor-stimulated cation currents in A7r5 smooth muscle cells. - Rat Genome Database

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TRPC6 is a candidate channel involved in receptor-stimulated cation currents in A7r5 smooth muscle cells.

Authors: Jung, S  Strotmann, R  Schultz, G  Plant, TD 
Citation: Jung S, etal., Am J Physiol Cell Physiol 2002 Feb;282(2):C347-59.
RGD ID: 619540
Pubmed: PMID:11788346   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpcell.00283.2001   (Journal Full-text)

To investigate the possible role of members of the mammalian transient receptor potential (TRP) channel family (TRPC1-7) in vasoconstrictor-induced Ca(2+) entry in vascular smooth muscle cells, we studied [Arg(8)]-vasopressin (AVP)-activated channels in A7r5 aortic smooth muscle cells. AVP induced an increase in free cytosolic Ca(2+) concentration ([Ca(2+)](i)) consisting of Ca(2+) release and Ca(2+) influx. Whole cell recordings revealed the activation of a nonselective cation current with a doubly rectifying current-voltage relation strikingly similar to those described for some heterologously expressed TRPC isoforms. The current was also stimulated by direct activation of G proteins as well as by activation of the phospholipase Cgamma-coupled platelet-derived growth factor receptor. Currents were not activated by store depletion or increased [Ca(2+)](i). Application of 1-oleoyl-2-acetyl-sn-glycerol stimulated the current independently of protein kinase C, a characteristic property of the TRPC3/6/7 subfamily. Like TRPC6-mediated currents, cation currents in A7r5 cells were increased by flufenamate. Northern hybridization revealed mRNA coding for TRPC1 and TRPC6. We therefore suggest that TRPC6 is a molecular component of receptor-stimulated Ca(2+)-permeable cation channels in A7r5 smooth muscle cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Trpc6Ratpositive regulation of cytosolic calcium ion concentration  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Trpc6  (transient receptor potential cation channel, subfamily C, member 6)


Additional Information