RGD Reference Report - Expression of cathepsin H in differentiating rat spermatids: immunoelectron microscopic study. - Rat Genome Database

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Expression of cathepsin H in differentiating rat spermatids: immunoelectron microscopic study.

Authors: Haraguchi, CM  Ishido, K  Kominami, E  Yokota, S 
Citation: Haraguchi CM, etal., Histochem Cell Biol. 2003 Jul;120(1):63-71. Epub 2003 Jul 1.
RGD ID: 5686399
Pubmed: PMID:12838426   (View Abstract at PubMed)
DOI: DOI:10.1007/s00418-003-0545-0   (Journal Full-text)

The expression of cathepsin H (CH) in differentiating rat spermatids was studied by an immunoelectron microscopic technique. Cathepsin H was detected in the acrosome throughout differentiation steps but cathepsins B, D, and L and lysosomal membrane protein (LGP107) were not. Early in the formation of the acrosome, CH signals were observed in Golgi vesicles but not in acrosomal vesicles. At steps 3-4, CH signals were associated with a fibrous material attached to the inner surface of the vesicle membrane on the Golgi side. At steps 5-6, this fibrous material accumulated to form an electron-dense sheet to which CH signals were confined. The rest of the acrosome was negative for the enzyme. At steps 11-12, the CH-positive fibrous sheet expanded from the apical to the ventral side of the sperm head. After step 16, the surface of outer dense fibers in the flagellar axoneme and reticulated bodies were stained for CH. In epididymal sperm, CH signals were detected in the acrosome as well as on the surface of the outer dense fibers running from the middle to the principal piece. By immunofluorescence staining, CH was found to be localized to the acrosome, middle piece, and principal piece.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CtshRatacrosomal vesicle  IDA  RGD 
CtshRataxoneme  IDA  RGD 
CtshRatouter dense fiber  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ctsh  (cathepsin H)


Additional Information