RGD Reference Report - Processing-dependent trafficking of Sonic hedgehog to the regulated secretory pathway in neurons. - Rat Genome Database

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Processing-dependent trafficking of Sonic hedgehog to the regulated secretory pathway in neurons.

Authors: Beug, ST  Parks, RJ  McBride, HM  Wallace, VA 
Citation: Beug ST, etal., Mol Cell Neurosci. 2011 Mar;46(3):583-96. Epub 2010 Dec 21.
RGD ID: 5509862
Pubmed: PMID:21182949   (View Abstract at PubMed)
DOI: DOI:10.1016/j.mcn.2010.12.009   (Journal Full-text)

Neurons are an important source of the secreted morphogen Sonic hedgehog (Shh), however, little is known about neuron-specific regulation of Shh transport and secretion. To study this process, we investigated the subcellular distribution of Shh in primary neurons and differentiated cells of a neuroendocrine cell line by fluorescence microscopy and biochemical fractionation. In retinal ganglion cells, endogenous Shh was distributed as intra- and extracellular puncta at the soma, dendrites, axons and neurite terminals. Shh(+) puncta move bidirectionally and colocalize with markers of synaptic vesicles (SVs) and dense core granules. Lipid modification and proteolysis were required for Shh sorting to SVs and cell surface association. Finally, consistent with its association with regulated secretory vesicles, Shh secretion could be induced under depolarizing conditions. Taken together, these observations suggest that long-range Shh transport and signalling in neurons involves trafficking to the regulated secretory pathway and cell surface accumulation of Shh on axons and suggests a link between neuronal activity and Shh release.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ShhRataxon  IDA retinal ganglion cells RGCRGD 
ShhRatdendrite  IDA retinal ganglion cells RGCRGD 
ShhRatextracellular space  IDA retinal ganglion cells RGCRGD 
ShhRatneuronal cell body  IDA retinal ganglion cells RGCRGD 

Objects Annotated

Genes (Rattus norvegicus)
Shh  (sonic hedgehog signaling molecule)


Additional Information