RGD Reference Report - Characterization of pyrimidine nucleoside monophosphokinase in normal and malignant tissues. - Rat Genome Database

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Characterization of pyrimidine nucleoside monophosphokinase in normal and malignant tissues.

Authors: Arima, T  Akiyoshi, H  Fujii, S 
Citation: Arima T, etal., Cancer Res. 1977 Jun;37(6):1593-7.
RGD ID: 5133256
Pubmed: PMID:192455   (View Abstract at PubMed)

It was found that there are two kinds of pyrimidine nucleoside, monophosphokinase deoxythymidine 5'-monophosphate-deoxyuridine 5'-monophosphate (dTMP-dUMP) kinase and cytidine 5'-monophosphate-deoxycytidine 5'-monophosphate-uridine 5'-monophosphate-doexyuridine 5'-monophosphate (CMP-dCMP-UMP-dUMP) kinase, and their molecular weights were calculated to be 46,000 and 26,000, respectively, by gel filtration. dTMP-dUMP kinase phosphorylated dTMP with a Km of 3.1 X 10(-5)M and dUMP with a Km of 7.7 X 10(-4) M. dTMP phosphorylation catalyzed by dTMP-dUMP kinase was inhibited competively by dUMP with a Ki of 2.0 X 10(-3) M. Similarly, phosphorylation of dUMP by this enzyme was inhibited competively by dTMP with a Ki of 2.5 X 10 (-5) M. CMP-dCMP-UMP-dUMP kinase of Yoshida sarcoma phosphorylated dUMP with a Km of 3.1 X 10(-3) M and dCMP with a Km of 7.1 X 10 (-4) M, but it did not phosphorylate dTMP. Phosphorylation of dUMP BY CMP-dCMP-UMP-dUMP kinase was inhibited competitively by DCMP and dTMP with Ki's of 6.9 X 10(-4) and 3.0 X 10(-3) M, respectively, and phosphorylation of dCMP was inhibited completely by dUMP a Ki of 2.2 X 10(-3) M. Relative Vmax activity of this enzyme was 345 nmoles/mg protein with dCMP and 127 nmoles/mg protein with dUMP.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cmpk1RatdCDP biosynthetic process  IDA  RGD 
Cmpk2RatdTDP biosynthetic process  IDA  RGD 
Cmpk2RatdUDP biosynthetic process  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cmpk2Ratthymidylate kinase activity  IDA  RGD 
Cmpk2RatUMP/dUMP kinase activity  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Objects Annotated

Genes (Rattus norvegicus)
Cmpk1  (cytidine/uridine monophosphate kinase 1)
Cmpk2  (cytidine/uridine monophosphate kinase 2)

Genes (Mus musculus)
Cmpk2  (cytidine/uridine monophosphate kinase 2)

Genes (Homo sapiens)
CMPK2  (cytidine/uridine monophosphate kinase 2)


Additional Information