RGD Reference Report - Characterization of pyrimidine nucleoside monophosphokinase in normal and malignant tissues. - Rat Genome Database

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Characterization of pyrimidine nucleoside monophosphokinase in normal and malignant tissues.

Authors: Arima, T  Akiyoshi, H  Fujii, S 
Citation: Arima T, etal., Cancer Res. 1977 Jun;37(6):1593-7.
RGD ID: 5133256
Pubmed: PMID:192455   (View Abstract at PubMed)

It was found that there are two kinds of pyrimidine nucleoside, monophosphokinase deoxythymidine 5'-monophosphate-deoxyuridine 5'-monophosphate (dTMP-dUMP) kinase and cytidine 5'-monophosphate-deoxycytidine 5'-monophosphate-uridine 5'-monophosphate-doexyuridine 5'-monophosphate (CMP-dCMP-UMP-dUMP) kinase, and their molecular weights were calculated to be 46,000 and 26,000, respectively, by gel filtration. dTMP-dUMP kinase phosphorylated dTMP with a Km of 3.1 X 10(-5)M and dUMP with a Km of 7.7 X 10(-4) M. dTMP phosphorylation catalyzed by dTMP-dUMP kinase was inhibited competively by dUMP with a Ki of 2.0 X 10(-3) M. Similarly, phosphorylation of dUMP by this enzyme was inhibited competively by dTMP with a Ki of 2.5 X 10 (-5) M. CMP-dCMP-UMP-dUMP kinase of Yoshida sarcoma phosphorylated dUMP with a Km of 3.1 X 10(-3) M and dCMP with a Km of 7.1 X 10 (-4) M, but it did not phosphorylate dTMP. Phosphorylation of dUMP BY CMP-dCMP-UMP-dUMP kinase was inhibited competitively by DCMP and dTMP with Ki's of 6.9 X 10(-4) and 3.0 X 10(-3) M, respectively, and phosphorylation of dCMP was inhibited completely by dUMP a Ki of 2.2 X 10(-3) M. Relative Vmax activity of this enzyme was 345 nmoles/mg protein with dCMP and 127 nmoles/mg protein with dUMP.




Biological Process

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Cmpk1RatdCDP biosynthetic process  IDA  RGD 
Cmpk2RatdTDP biosynthetic process  IDA  RGD 
Cmpk1RatdUDP biosynthetic process  IDA  RGD 
Cmpk2RatdUDP biosynthetic process  IDA  RGD 

Molecular Function

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Cmpk1Rat(d)CMP kinase activity  IDA  RGD 
Cmpk2RatdTMP kinase activity  IDA  RGD 
Cmpk1RatdUMP kinase activity  IDA  RGD 
Cmpk2RatdUMP kinase activity  IDA  RGD 
Cmpk1RatUMP kinase activity  IDA  RGD 

RGD Manual Annotations


  

Genes (Rattus norvegicus)
Cmpk1  (cytidine/uridine monophosphate kinase 1) Cmpk2  (cytidine/uridine monophosphate kinase 2)

Genes (Mus musculus)
Cmpk2  (cytidine/uridine monophosphate kinase 2)

Genes (Homo sapiens)
CMPK2  (cytidine/uridine monophosphate kinase 2)