RGD Reference Report - Increased CD56(bright) NK cells in HIV-HCV co-infection and HCV mono-infection are associated with distinctive alterations of their phenotype. - Rat Genome Database

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Increased CD56(bright) NK cells in HIV-HCV co-infection and HCV mono-infection are associated with distinctive alterations of their phenotype.

Authors: Bhardwaj, Suvercha  Ahmad, Fareed  Wedemeyer, Heiner  Cornberg, Marcus  Schulze Zur Wiesch, Julian  van Lunzen, Jan  Sarin, Shiv K  Schmidt, Reinhold E  Meyer-Olson, Dirk 
Citation: Bhardwaj S, etal., Virol J. 2016 Apr 18;13:67. doi: 10.1186/s12985-016-0507-5.
RGD ID: 40818300
Pubmed: PMID:27091211   (View Abstract at PubMed)
PMCID: PMC4835889   (View Article at PubMed Central)
DOI: DOI:10.1186/s12985-016-0507-5   (Journal Full-text)


BACKGROUND: HIV-HCV co-infection is associated with accelerated progression to hepatic fibrosis, cirrhosis and hepatocellular carcinoma than HCV mono-infection. The contribution of innate immunity during HIV-HCV co-infection has been a relatively under-investigated area. Natural killer (NK) cells are pivotal sentinels of innate immunity against viruses and tumour cells. In this study we evaluated the effect of HIV-HCV co-infection on peripheral blood NK cell subsets with emphasis on the phenotype of CD56(bright) NK cells.
METHODS: Sixty patients were included in the study; HIV mono-infected (n = 12), HCV mono-infected (n = 15), HCV-HIV co-infected (n = 21) and healthy controls (n = 16). PBMCs were isolated and immunophenotyping of NK cells was performed by flowcytometry.
RESULTS: We observed an expansion of CD56(bright) NK cell subset in HIV-HCV co-infection as compared to healthy controls and HIV mono-infected group. All the infected groups had an upregulated expression of the activating receptor NKG2D on CD56(bright) NK cells in comparison to healthy controls while not differing amongst themselves. The expression of NKp46 in HIV-HCV co-infected group was significantly upregulated as compared to both HIV as well as HCV mono-infections while NKp30 expression in the HIV-HCV co-infected group significantly differed as compared to HIV mono-infection. The CD56(bright) NK cell subset was activated in HIV-HCV co-infection as assessed by the expression of CD69 as compared to healthy controls but was significantly downregulated in comparison to HIV mono-infection. CD95 expression on CD56(bright) NK cells followed the same pattern where there was an increased expression of CD95 in HIV mono-infection and HIV-HCV co-infection as compared to healthy controls. In contrast to CD69 expression, CD95 expression in HCV mono-infection was decreased when compared to HIV mono-infection and HIV-HCV co-infection. Finally, expression of CXCR3 on CD56(bright) NK cells was increased in HIV-HCV co-infection in comparison to HIV mono-infection while remaining similar to HCV mono-infection.
CONCLUSION: Thus, HIV-HCV co-infection is able to modulate the phenotype of CD56(bright) NK cell subset in a unique way such that NKp46 and CXCR3 expressions are distinct for co-infection while both mono-infections have an additive effect on CD56(bright), CD69 with CD95 expressions. HCV mono-infection has a dominant effect on NKp30 expression while NKG2D and CD127 expressions remained same in all the groups.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
KLRK1Humanhepatitis C disease_progressionIEP associated with HIV Seropositivity more ...RGD 
KLRK1Humanhepatitis C  IEP protein:increased expression:peripheral blood mononuclear cell and natural killer cell (human)RGD 
Klrk1Rathepatitis C disease_progressionISOKLRK1 (Homo sapiens)associated with HIV Seropositivity more ...RGD 
Klrk1Mousehepatitis C  ISOKLRK1 (Homo sapiens)protein:increased expression:peripheral blood mononuclear cell and natural killer cell (human)RGD 
Klrk1Mousehepatitis C disease_progressionISOKLRK1 (Homo sapiens)associated with HIV Seropositivity more ...RGD 
Klrk1Rathepatitis C  ISOKLRK1 (Homo sapiens)protein:increased expression:peripheral blood mononuclear cell and natural killer cell (human)RGD 
KLRK1HumanHIV Seropositivity  IEP protein:increased expression:peripheral blood mononuclear cell and natural killer cell (human)RGD 
Klrk1MouseHIV Seropositivity  ISOKLRK1 (Homo sapiens)protein:increased expression:peripheral blood mononuclear cell and natural killer cell (human)RGD 
Klrk1RatHIV Seropositivity  ISOKLRK1 (Homo sapiens)protein:increased expression:peripheral blood mononuclear cell and natural killer cell (human)RGD 
NCR1HumanHIV Seropositivity  IEP protein:decreased expression:peripheral blood mononuclear cell and natural killer cell (human)RGD 
Ncr1RatHIV Seropositivity  ISONCR1 (Homo sapiens)protein:decreased expression:peripheral blood mononuclear cell and natural killer cell (human)RGD 
Ncr1MouseHIV Seropositivity  ISONCR1 (Homo sapiens)protein:decreased expression:peripheral blood mononuclear cell and natural killer cell (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Klrk1  (killer cell lectin like receptor K1)
Ncr1  (natural cytotoxicity triggering receptor 1)

Genes (Mus musculus)
Klrk1  (killer cell lectin-like receptor subfamily K, member 1)
Ncr1  (natural cytotoxicity triggering receptor 1)

Genes (Homo sapiens)
KLRK1  (killer cell lectin like receptor K1)
NCR1  (natural cytotoxicity triggering receptor 1)


Additional Information