RGD Reference Report - The subcellular distribution of GABARAP and its ability to interact with NSF suggest a role for this protein in the intracellular transport of GABA(A) receptors. - Rat Genome Database

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The subcellular distribution of GABARAP and its ability to interact with NSF suggest a role for this protein in the intracellular transport of GABA(A) receptors.

Authors: Kittler, J T  Rostaing, P  Schiavo, G  Fritschy, J M  Olsen, R  Triller, A  Moss, S J 
Citation: Kittler JT, etal., Mol Cell Neurosci. 2001 Jul;18(1):13-25. doi: 10.1006/mcne.2001.1005.
RGD ID: 405650362
Pubmed: PMID:11461150   (View Abstract at PubMed)
DOI: DOI:10.1006/mcne.2001.1005   (Journal Full-text)

GABA(A) receptors the major sites of fast synaptic inhibition in the brain are composed predominately of alpha, beta, and gamma2 subunits. The receptor gamma2 subunit interacts with a 17-kDa microtubule associated protein GABARAP, but the significance of this interaction remains unknown. Here we demonstrate that GABARAP, which immunoprecipitates with GABA(A) receptors, is not found at significant levels within inhibitory synapses, but is enriched within the Golgi apparatus and postsynaptic cisternae. We also demonstrate that GABARAP binds directly to N-ethylmaleimide-sensitive factor (NSF), a protein critical for intracellular membrane trafficking events. NSF and GABARAP complexes could be detected in neurons and these two proteins also colocalize within intracellular membrane compartments. Together our observations suggest that GABARAP may play a role in intracellular GABA(A) receptor transport but not synaptic anchoring, via its ability to interact with NSF. GABARAP may therefore have an important role in the production of GABAergic synapses.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GabarapRatGABA-ergic synapse is_active_inIDA PMID:11461150SynGO 
NsfRatGABA-ergic synapse is_active_inIDA PMID:11461150SynGO 
GabarapRatpostsynaptic specialization, intracellular component is_active_inIDA PMID:11461150SynGO 
NsfRatpostsynaptic specialization, intracellular component is_active_inIDA PMID:11461150SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Gabarap  (GABA type A receptor-associated protein)
Nsf  (N-ethylmaleimide sensitive factor, vesicle fusing ATPase)


Additional Information