RGD Reference Report - Effects of mu opioid receptors in paraventricular nucleus on ejaculation through mediating sympathetic nerve system activity. - Rat Genome Database

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Effects of mu opioid receptors in paraventricular nucleus on ejaculation through mediating sympathetic nerve system activity.

Authors: Zhang, Qi-Jie  Yang, Jie  Wang, Ya-Min  Cong, Rong  Zhu, Guo-Qing  Wang, Zeng-Jun  Song, Ning-Hong  Xia, Jia-Dong 
Citation: Zhang QJ, etal., Neuropharmacology. 2019 Nov 1;158:107709. doi: 10.1016/j.neuropharm.2019.107709. Epub 2019 Jul 13.
RGD ID: 401831048
Pubmed: PMID:31310777   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neuropharm.2019.107709   (Journal Full-text)

To investigate the roles of mu opioid receptors (MORs) in paraventricular nucleus of the hypothalamus (PVN) on ejaculation and its underlying mechanism in the rats, we performed copulation behavioral testing and acute experiments. During the acute experiments, mean arterial pressure (MAP), heart rate (HR), bulbospongiosus muscle-electromyogram (BSM-EMG) and pressure of vas deferens (PVD) were all recorded. The expression levels and distributions of opioid receptors were also assessed in PVN of male rats. Moreover, adeno-associated virus type 1 (AAV1) was microinjected into PVN to demonstrate whether there are direct projections from PVN to lumbar spinothalamic (LSt) cells. We found that microinjection of MOR agonist, D-A1a2-NM9-Phe4-Gly(ol)5enkephalin (DAGO), into the PVN prolonged the intromission latency and inhibited ejaculation (P = 0.0241, P = 0.0473, respectively), while the opposed results appeared in CTAP (D-Phe-Cys-Tyr-D-Trp-Arg-Thr-Pen-Thr-NH2, MOR antagonist) group (P = 0.0021, P = 0.0286, respectively). Moreover, DAGO caused a significant decrease in MAP and HR (P = 0.0065, P = 0.0030, respectively), and PVD decreased significantly after DAGO microinjection in PVN (P = 0.0383). CTAP not only blocked the effect of DAGO but also significantly increased MAP, HR and PVD (P = 0.0003, P = 0.0010, P = 0.0074, respectively). Meanwhile, a significant increase was observed in BSM-EMG activity after microinjecting of CTAP (P = 0.0022), accompanied by visible BSM contraction. Additionally, anterograde monosynaptic transneuronal tracer AAV1 labeling revealed that neurons in PVN projected directly to LSt cells in L3-4 spinal cord. These results indicate that MORs in PVN centrally mediate ejaculation by regulating the sympathetic outflow, which may be treated as a therapeutic target for ejaculation disorders in the future.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Oprm1Ratsperm ejaculation  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Oprm1  (opioid receptor, mu 1)


Additional Information