RGD Reference Report - The nerve growth factor-responsive PC12 cell line does not express the Myc dimerization partner Max. - Rat Genome Database

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The nerve growth factor-responsive PC12 cell line does not express the Myc dimerization partner Max.

Authors: Hopewell, R  Ziff, EB 
Citation: Hopewell R and Ziff EB, Mol Cell Biol. 1995 Jul;15(7):3470-8.
RGD ID: 2316831
Pubmed: PMID:7791753   (View Abstract at PubMed)
PMCID: PMC230583   (View Article at PubMed Central)

Heterodimerization of Max with the nuclear oncoprotein Myc and the differentiation-associated proteins Mad and Mxi1 enables these factors to bind E-box sites in DNA and control genes implicated in cell proliferation and differentiation. We show that in the PC12 pheochromocytoma tumor cell line, functional Max protein is not expressed because of the synthesis of a mutant max transcript. This transcript encodes a protein incapable of homo- or heterodimerization. Furthermore, the mutant Max protein, unlike wild-type Max, is incapable of repressing transcription from an E-box element. Synthesis of mutant max transcripts appears to be due to a homozygous chromosomal alteration within the max gene. Reintroduction of max into PC12 cells results in repression of E-box-dependent transcription and a reduction in growth rate, which may explain the loss of Max expression either during the growth of the pheochromocytoma or in subsequent passage of the PC12 cell line in vitro. Finally, the ability of these cells to divide, differentiate, and apoptose in the absence of Max demonstrates for the first time that these processes can occur via Max- and possibly Myc-independent mechanisms.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MaxRatnegative regulation of gene expression  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MaxRatidentical protein binding  IPIMax (Rattus norvegicus)homodimerizationRGD 
MaxRatprotein-containing complex binding NOTIDA heterodimerization and truncated variantRGD 

Objects Annotated

Genes (Rattus norvegicus)
Max  (MYC associated factor X)


Additional Information