RGD Reference Report - Ultrastructural evidence for co-localization of corticotropin-releasing factor receptor and mu-opioid receptor in the rat nucleus locus coeruleus. - Rat Genome Database

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Ultrastructural evidence for co-localization of corticotropin-releasing factor receptor and mu-opioid receptor in the rat nucleus locus coeruleus.

Authors: Reyes, BA  Glaser, JD  Van Bockstaele, EJ 
Citation: Reyes BA, etal., Neurosci Lett. 2007 Feb 21;413(3):216-21. Epub 2006 Dec 15.
RGD ID: 2316621
Pubmed: PMID:17194545   (View Abstract at PubMed)
PMCID: PMC1839951   (View Article at PubMed Central)
DOI: DOI:10.1016/j.neulet.2006.11.069   (Journal Full-text)

Previous studies have shown that corticotropin-releasing factor (CRF), an integral mediator of the stress response, and opioids regulate the activity of the locus-coeruleus-norepinephrine (LC-NE) system during stress in a reciprocal manner. Furthermore, repeated opiate exposure sensitizes noradrenergic neurons to CRF. Previous studies have shown that mu-opioid receptors (muORs) are prominently distributed within somatodendritic processes of catecholaminergic neurons in the LC and axon terminals containing opioid peptides and CRF converge within the LC. To further examine cellular sites for interactions between CRF receptor type 1 (CRFr) and muOR, immunofluorescence and electron microscopic analysis of the rat LC was conducted. Triple immunofluorescence showed prominent co-localization of the CRFr and muOR in noradrenergic somata in the LC. Ultrastructural analysis confirmed dual localization of CRFr and muOR in common dendritic processes in the LC. Semi-quantitative analysis showed that of the dendrites exhibiting CRFr immunolabeling, 57% expressed muOR immunoreactivity. These data provide ultrastructural evidence that CRFr and muOR are co-localized in LC neurons, a cellular substrate that may underlie opiate-induced sensitization of brain noradrenergic neurons to CRF.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Oprm1Ratdendrite  IDA  RGD 
Oprm1Ratperikaryon  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Oprm1  (opioid receptor, mu 1)


Additional Information