RGD Reference Report - Overexpression of acyl-coA binding protein and its effects on the flux of free fatty acids in McA-RH 7777 cells. - Rat Genome Database

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Overexpression of acyl-coA binding protein and its effects on the flux of free fatty acids in McA-RH 7777 cells.

Authors: Yang, Y  Pritchard, PH  Bhuiyan, J  Seccombe, DW  Moghadasian, MH 
Citation: Yang Y, etal., Lipids. 2001 Jun;36(6):595-600.
RGD ID: 2316274
Pubmed: PMID:11485163   (View Abstract at PubMed)

Overexpression of acyl-CoA binding protein (ACBP) was induced in a rat hepatoma cell line (McA-RH 7777) by stable integration of rat ACBP cDNA. The transfected cells (ACBP-27) had 3.5-fold higher concentrations of ACBP than control cells (14 vs. 4 ng/microg DNA). Both ACBP-27 and control cells were cultured in the presence of various concentrations of radiolabeled palmitic acid; and the effects of ACBP on lipogenesis and beta-oxidation were studied. Incubation of the cells with 100 microM palmitic acid resulted in 42% greater incorporation of the fatty acid in ACBP-27 cells as compared to that in the control cells. This increased incorporation of the fatty acid was observed predominantly in the triglyceride fraction. Higher concentrations of palmitic acid (200 to 400 microM) were associated with a significant decrease in the production of 14CO2 in the ACBP-27 cell line than in the control cells, while lower concentrations had no effect. Our data suggest a role for ACBP in the partitioning of fatty acids between esterification reactions leading to the formation of neutral lipids and beta-oxidation. ACBP may play a regulatory role by influencing this important branch point in intermediary lipid metabolism.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
DbiRatnegative regulation of fatty acid beta-oxidation  IDA  RGD 
DbiRatpositive regulation of lipid biosynthetic process  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dbi  (diazepam binding inhibitor)


Additional Information