RGD Reference Report - Expression analysis and study of KLK4 in benign and malignant breast tumours. - Rat Genome Database

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Expression analysis and study of KLK4 in benign and malignant breast tumours.

Authors: Papachristopoulou, G  Avgeris, M  Scorilas, A 
Citation: Papachristopoulou G, etal., Thromb Haemost. 2009 Feb;101(2):381-7.
RGD ID: 2314852
Pubmed: PMID:19190825   (View Abstract at PubMed)

The steroid hormone-regulated gene KLK4 (kallikrein 4) is a new member of the human kallikrein-related peptidase gene family. Up to date, studies report that KLK4 is differentially expressed in many tumours. The purpose of this study was the expression analysis and study of KLK4 in benign and malignant breast tumours. Total RNA was isolated from 16 benign and 45 malignant breast tissue specimens. After testing RNA quality, cDNA was prepared by reverse transcription. Highly sensitive quantitative real-time PCR method for KLK4 mRNA quantification was developed using the SYBR Green chemistry. GAPDH served as a housekeeping gene. Relative quantification analysis was performed using the comparative C(T) method 2(-DeltaDeltaC)(T) KLK4 expression was found to vary in both patients' cohorts; however, a statistically significant elevation of the KLK4 mRNA levels was observed in malignant compared to benign tumour patients. Low KLK4 expression levels were found in well-differentiated tumours (p = 0.011) as well as in stage I (p = 0.024) patients. Moreover, a statistically significant (r(s) = -0.318, p = 0.035) negative correlation between the KLK4 expression and progesterone receptor staining was observed. ROC and logistic regression analysis recommended that KLK4 gene expression may be used as a new potential biomarker in breast cancer.




  
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Original Reference(s)
KLK4Humanbreast cancer severityIEP  RGD 
Klk4Ratbreast cancer severityISORGD:1353266 RGD 
Klk4Mousebreast cancer severityISORGD:1353266 RGD 


Genes (Rattus norvegicus)
Klk4  (kallikrein-related peptidase 4)

Genes (Mus musculus)
Klk4  (kallikrein related-peptidase 4 (prostase, enamel matrix, prostate))

Genes (Homo sapiens)
KLK4  (kallikrein related peptidase 4)