RGD Reference Report - Epithelial to mesenchymal transformation in tubular epithelial cells undergoing anoxia. - Rat Genome Database

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Epithelial to mesenchymal transformation in tubular epithelial cells undergoing anoxia.

Authors: Luo, GH  Lu, YP  Yang, L  Song, J  Shi, YJ  Li, YP 
Citation: Luo GH, etal., Transplant Proc. 2008 Oct;40(8):2800-3.
RGD ID: 2314608
Pubmed: PMID:18929865   (View Abstract at PubMed)
DOI: DOI:10.1016/j.transproceed.2008.08.004   (Journal Full-text)

AIM: Epithelial-mesenchymal transformation (EMT) has been proved to be a critical event in fibrogenesis of renal allografts. This study sought to determine whether anoxia could induce EMT from tubular epithelial cells (TEC). METHODS: Rat TEC-line (NRK-52E) was cultured in Dulbelco's modified Eagle's medium (DMEM) without glucose under 100% N2 for 4 hours. After 6, 12, 24, 48, and 72 hours, the expressions of connective tissue growth factor (CTGF) mRNA and protein were measured by real-time RT-PCR and Western blot, respectively. Morphologic changes and cytoskeleton remodeling were observed in NRK-52E cells under laser confocal microscopy. Immunohistochemistry and flow cytometry were used to detect expression changes of E-cadherin, alpha-smooth muscle actin (SMA), types I and IV collagen, all of which are involved in TEC, EMT. RESULTS: After stimulation by anoxia, NRK-52E cells became round and enlarged with a remodeled cytoskeleton. The expressions of CTGF mRNA and protein were upregulated after 6 hours, reaching their peak at 48 hours. The expressions of types I and IV collagen, and alpha-SMA were all upregulated except for E-cadherin. CONCLUSIONS: Anoxia upregulated the expression of CTGF and other EMT-associated genes in NRK-52E cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ccn2Ratresponse to anoxia  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ccn2  (cellular communication network factor 2)


Additional Information