RGD Reference Report - Characterization of a mammalian Golgi-localized protein complex, COG, that is required for normal Golgi morphology and function. - Rat Genome Database

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Characterization of a mammalian Golgi-localized protein complex, COG, that is required for normal Golgi morphology and function.

Authors: Ungar, D  Oka, T  Brittle, EE  Vasile, E  Lupashin, VV  Chatterton, JE  Heuser, JE  Krieger, M  Waters, MG 
Citation: Ungar D, etal., J Cell Biol. 2002 Apr 29;157(3):405-15. Epub 2002 Apr 29.
RGD ID: 2313611
Pubmed: PMID:11980916   (View Abstract at PubMed)
PMCID: PMC2173297   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.200202016   (Journal Full-text)

Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Three complexes that have previously been partially characterized include (a) the Golgi transport complex (GTC), identified in an in vitro membrane transport assay, (b) the ldlCp complex, identified in analyses of CHO cell mutants with defects in Golgi-associated glycosylation reactions, and (c) the mammalian Sec34 complex, identified by homology to yeast Sec34p, implicated in vesicular transport. We show that these three complexes are identical and rename them the conserved oligomeric Golgi (COG) complex. The COG complex comprises four previously characterized proteins (Cog1/ldlBp, Cog2/ldlCp, Cog3/Sec34, and Cog5/GTC-90), three homologues of yeast Sec34/35 complex subunits (Cog4, -6, and -8), and a previously unidentified Golgi-associated protein (Cog7). EM of ldlB and ldlC mutants established that COG is required for normal Golgi morphology. "Deep etch" EM of purified COG revealed an approximately 37-nm-long structure comprised of two similarly sized globular domains connected by smaller extensions. Consideration of biochemical and genetic data for mammalian COG and its yeast homologue suggests a model for the subunit distribution within this complex, which plays critical roles in Golgi structure and function.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cog2Ratprotein-containing complex binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cog2  (component of oligomeric golgi complex 2)


Additional Information