RGD Reference Report - Aquaporin-2 trafficking is regulated by PDZ-domain containing protein SPA-1. - Rat Genome Database

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Aquaporin-2 trafficking is regulated by PDZ-domain containing protein SPA-1.

Authors: Noda, Y  Horikawa, S  Furukawa, T  Hirai, K  Katayama, Y  Asai, T  Kuwahara, M  Katagiri, K  Kinashi, T  Hattori, M  Minato, N  Sasaki, S 
Citation: Noda Y, etal., FEBS Lett. 2004 Jun 18;568(1-3):139-45.
RGD ID: 2313141
Pubmed: PMID:15196935   (View Abstract at PubMed)
DOI: DOI:10.1016/j.febslet.2004.05.021   (Journal Full-text)

Targeted positioning of water channel aquaporin-2 (AQP2) strictly regulates body water homeostasis. Trafficking of AQP2 to the apical membrane is critical to the reabsorption of water in renal collecting ducts. Controlled apical positioning of AQP2 suggests the existence of proteins that interact with AQP2. A biochemical search for AQP2-interacting proteins led to the identification of PDZ-domain containing protein, signal-induced proliferation-associated gene-1 (SPA-1) which is a GTPase-activating protein (GAP) for Rap1. The distribution of SPA-1 coincided with that of AQP2 in renal collecting ducts. The site of colocalization was concomitantly relocated by hydration status. AQP2 trafficking to the apical membrane was inhibited by the SPA-1 mutant lacking Rap1GAP activity and by the constitutively active mutant of Rap1. AQP2 trafficking was impaired in SPA-1-deficient mice. Our results show that SPA-1 directly binds to AQP2 and regulates at least in part AQP2 trafficking.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aqp2Ratcellular response to water deprivation  IDA  RGD 
Sipa1Ratcellular response to water deprivation  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aqp2Ratprotein-containing complex  IDA  RGD 
Sipa1Ratprotein-containing complex  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aqp2RatPDZ domain binding  IPISipa1 (Rattus norvegicus) RGD 
Sipa1Ratprotein binding  IPIAqp2 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Aqp2  (aquaporin 2)
Sipa1  (signal-induced proliferation-associated 1)


Additional Information