RGD Reference Report - Regulation of intestinal NaPi-IIb cotransporter gene expression by estrogen. - Rat Genome Database

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Regulation of intestinal NaPi-IIb cotransporter gene expression by estrogen.

Authors: Xu, H  Uno, JK  Inouye, M  Xu, L  Drees, JB  Collins, JF  Ghishan, FK 
Citation: Xu H, etal., Am J Physiol Gastrointest Liver Physiol. 2003 Dec;285(6):G1317-24. Epub 2003 Jul 31.
RGD ID: 2311315
Pubmed: PMID:12893629   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpgi.00172.2003   (Journal Full-text)

The current experiments were designed to study the effect of beta-estradiol on type IIb sodium-coupled phosphate (NaPi-IIb) cotransporter gene expression. Uptake studies with intestinal brush-border membrane vesicles (BBMV) showed that estrogen treatment increased sodium-dependent phosphate absorption by approximately 45% in rat intestine. Northern blot analysis indicated that NaPi-IIb mRNA expression was increased by approximately 50% after estrogen treatment. Western blot analysis also detected an increase in BBMV NaPi-IIb protein expression in estrogen-treated rats. In human intestinal Caco-2 cells, NaPi-IIb mRNA abundance was increased approximately 60% after estrogen treatment, and this increase could be abolished by inhibition of gene transcription. Transfection studies with human NaPi-IIb promoter reporter constructs showed that the promoter was responsive to estrogen treatment. These studies demonstrate for the first time that estrogen stimulates intestinal sodium-dependent phosphate absorption in female rats. This stimulation is associated with increased NaPi-IIb mRNA and protein expression. Thus the effect of estrogen on intestinal Pi absorption may be partially due to activation of NaPi-IIb gene transcription.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc34a2Ratresponse to estradiol  IEP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc34a2Ratapical plasma membrane located_inIDA PMID:12893629UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc34a2Ratsodium:phosphate symporter activity enablesIDA PMID:12893629UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Slc34a2  (solute carrier family 34 member 2)


Additional Information