RGD Reference Report - Interaction of glyceraldehyde-3-phosphate dehydrogenase in the light-induced rod alpha-transducin translocation. - Rat Genome Database

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Interaction of glyceraldehyde-3-phosphate dehydrogenase in the light-induced rod alpha-transducin translocation.

Authors: Chen, J  Wu, M  Sezate, SA  Matsumoto, H  Ramsey, M  McGinnis, JF 
Citation: Chen J, etal., J Neurochem. 2008 Mar;104(5):1280-92. Epub 2007 Nov 17.
RGD ID: 2302128
Pubmed: PMID:18028335   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1471-4159.2007.05081.x   (Journal Full-text)

The light-dependent subcellular translocation of rod alpha-transducin (GNAT-1, or rod Talpha) has been well documented. In dark-adapted animals, rod Talpha (rTalpha) is predominantly located in the rod outer segment (ROS) and translocates into the rod inner segment (RIS) upon exposure to the light. Neither the molecular participants nor the mechanism(s) involved in this protein trafficking are known. We hypothesized that other proteins must interact with rTalpha to affect the translocations. Using the MBP-rTalpha fusion pulldown assay, the yeast two-hybrid assay and the co-immunoprecipitation assay, we identified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and rTalpha as interacting proteins. Immunoprecipitation also showed beta-actin associates with rTalpha in the dark but not in the light. To further investigate the involvement of GAPDH in light-induced rod Talpha translocation, GAPDH mRNA was knocked down in vivo by transient expression of siRNAs in rat photoreceptor cells. Under completely dark- and light-adapted conditions, the translocation of rTalpha was not significantly different within the 'GAPDH knock-down photoreceptor cells' compared to the non-transfected control cells. However, under partial dark-adaptation, rTalpha translocated more slowly in the 'GAPDH knock-down cells' supporting the conclusion that GAPDH is involved in rTalpha translocation from the RIS to the ROS during dark adaptation.




Molecular Function

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
GapdhRatprotein binding  IPIGnat1 (Rattus norvegicus) RGD 
Gnat1Ratprotein binding  IPIGapdh (Rattus norvegicus) RGD 


Genes (Rattus norvegicus)
Gapdh  (glyceraldehyde-3-phosphate dehydrogenase) Gnat1  (G protein subunit alpha transducin 1)