RGD Reference Report - The cytochemical demonstration of prostatic acid phosphatase using a new substrate, phosphorylcholine. - Rat Genome Database

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The cytochemical demonstration of prostatic acid phosphatase using a new substrate, phosphorylcholine.

Authors: Serrano, JA  Shannon WA, JR  Sternberger, NJ  Wasserkrug, HL  Serrano, AA  Seligman, AM 
Citation: Serrano JA, etal., J Histochem Cytochem. 1976 Oct;24(10):1046-56.
RGD ID: 2299977
Pubmed: PMID:977936   (View Abstract at PubMed)

Prostatic acid phosphatase (PAP), an acid phosphatase specific to the prostate gland, is demonstrated cytochemically for both light and electron microscopy with a new substrate phosphorylcholine. Lead ion is used as capture agent for liberated phosphate ion in a modified Gomori medium. PAP is demonstrated in the tubuloaveolar epithelial secretory cells of the rat ventral prostate gland. In the apical portion of the cell it is found in secretory granules and in the matrix of multivescular bodies. In the Golgi area it is localized in Golgi cisternae, Golgi related vacuoles and multivescular bodies. Evidence is presented that PAP is not a lysosomal enzyme, as are other acid phosphatases, and that phosphorylcholine is a highly specific substrate for PAP. As based on the role of pentavalent nitrogen on substrate structure, it is apparent that PAP is to other acid phosphatases what the cholinesterases are to other esterases.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Acp3Ratdephosphorylation  IDA phosphorylcholine substrateRGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Acp3Ratapical part of cell  IDA  RGD 
Acp3RatGolgi cisterna  IDA  RGD 
Acp3Ratmultivesicular body  IDA  RGD 
Acp3Ratsecretory granule  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Acp3Ratacid phosphatase activity  IDA phosphorylcholine substrateRGD 
Acp3Ratcholine binding  IDA phosphorylcholine substrateRGD 

Objects Annotated

Genes (Rattus norvegicus)
Acp3  (acid phosphatase 3)


Additional Information