RGD Reference Report - Expression and localization of inhibin alpha-subunit in rat retinal photoreceptor cells. - Rat Genome Database

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Expression and localization of inhibin alpha-subunit in rat retinal photoreceptor cells.

Authors: Ying, SY  Li, SQ  Zhang, Z 
Citation: Ying SY, etal., Life Sci. 1995;57(1):45-52.
RGD ID: 2290440
Pubmed: PMID:7596220   (View Abstract at PubMed)

To determine whether rat retinal photoreceptor cells produce inhibin, a molecule closely related to activin, a multifunctional growth factor in the transforming growth factor beta superfamily (TGF beta), we have conducted immunohistochemistry using specific antibodies for inhibin which were raised against a synthetic N-terminal fragment of the alpha-subunit of inhibin. The mature inhibin molecule was identified at both the inner and outer segments of photoreceptor cells. To determine if messenger RNA for the alpha-subunit of inhibin is expressed in the retinal cells, both in situ hybridization with a specific probe and the reverse transcription-polymerase chain reaction (RT-PCR) technique with primers specific for the alpha-subunit of inhibin were used. Messenger RNA expression of the alpha-subunit of inhibin was detected by RT-PCR and localized in the photoreceptor cells as determined by in situ hybridization. In addition, the identity of the cDNA product of RT-PCR was verified with Southern analysis and DNA sequencing. The localization of mature inhibin protein and its corresponding message to photoreceptor cells suggest that inhibin may have a paracrine function in the retina, perhaps in the photoreceptor cells themselves.




Cellular Component

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
InhaRatphotoreceptor inner segment  IDA  RGD 
InhaRatphotoreceptor outer segment  IDA  RGD 


Genes (Rattus norvegicus)
Inha  (inhibin subunit alpha)