RGD Reference Report - Brief episode of ischemia activates protective genetic program in rat heart: a gene chip study. - Rat Genome Database

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Brief episode of ischemia activates protective genetic program in rat heart: a gene chip study.

Authors: Simkhovich, BZ  Marjoram, P  Poizat, C  Kedes, L  Kloner, RA 
Citation: Simkhovich BZ, etal., Cardiovasc Res. 2003 Aug 1;59(2):450-9.
RGD ID: 2289075
Pubmed: PMID:12909328   (View Abstract at PubMed)

OBJECTIVE: Brief episodes of ischemia of 20 min or less have the potential to protect the heart. Such episodes are associated primarily with reversible ischemic injury yet they induce changes in gene expression. The purpose of the study was to determine whether activation of protective genes takes place within 4 h following a brief episode of ischemia that would mimic angina pectoris. METHODS: Three groups of rats were studied. In the control (Ctrl) group, hearts were immediately excised following anesthesia; in the sham-operated (SO) group, opened-chest rats received 4 h and 20 min of no intervention; and in the group subjected to ischemia (SI) hearts received 20 min of proximal coronary occlusion followed by 4 h of reperfusion. Hearts from the SI group were divided into nonischemic (NI) and ischemic (Isc) areas. Changes in gene expression pattern were analyzed by using Affymetrix Gene Chips. RESULTS: Ischemia led to strong upregulation of mRNA transcripts for heat shock proteins 70, 27, 105, 86 and 40 kDa, vascular endothelial growth factor, brain-derived neurotrophic factor, plasminogen activator inhibitor-1, activating transcription factor 3, B-cell translocation gene 2, and growth arrest and DNA damage inducible 45 alpha protein compared to the NI tissue. The majority of mRNAs whose levels increased following brief ischemia were of a protective nature. CONCLUSION: Genetic reprogramming emerging during or following brief episodes of ischemia that simulate angina, can be characterized as protective in nature. Developing new therapeutic strategies aimed to promote this protective response represents a legitimate target for future research.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Myocardial Reperfusion Injury  ISOBtg2 (Rattus norvegicus)2289075; 2289075mRNA:increased expression:heartRGD 
Myocardial Reperfusion Injury  IEP 2289075mRNA:increased expression:heartRGD 

Objects Annotated

Genes (Rattus norvegicus)
Btg2  (BTG anti-proliferation factor 2)

Genes (Mus musculus)
Btg2  (BTG anti-proliferation factor 2)

Genes (Homo sapiens)
BTG2  (BTG anti-proliferation factor 2)


Additional Information