RGD Reference Report - Insulin and cyclic AMP act at different levels on transcription of the L-type pyruvate kinase gene. - Rat Genome Database

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Insulin and cyclic AMP act at different levels on transcription of the L-type pyruvate kinase gene.

Authors: Cuif, MH  Doiron, B  Kahn, A 
Citation: Cuif MH, etal., FEBS Lett. 1997 Nov 3;417(1):81-4.
RGD ID: 1625596
Pubmed: PMID:9395079   (View Abstract at PubMed)

We previously demonstrated that, in hepatocytes in primary culture, the role of insulin on induction of L-type pyruvate kinase (L-PK) gene expression was mainly to induce glucokinase synthesis, needed for glucose phosphorylation to glucose 6-phosphate. However, we show here that when hepatocytes have been isolated from rats starved for 72 h, glucose and constitutive glucokinase expression was not sufficient to fully stimulate the L-PK promoter, low insulin concentrations being still required. In addition, activation remains sensitive to cAMP inhibition, but cannot be reproduced in the absence of insulin by a competitive cAMP antagonist. We propose that both insulin and cAMP act on expression of the L-PK gene at, at least, two levels: positive and negative regulation of glucokinase gene expression, and more downstream levels.




Biological Process

  
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Original Reference(s)
PklrRatcellular response to insulin stimulus  IDA  RGD 
PklrRatresponse to cAMP  IDA  RGD 


Genes (Rattus norvegicus)
Pklr  (pyruvate kinase L/R)