RGD Reference Report - Subcellular fractionation of liver organelles from phenobarbital-treated rats by counter-current partition and sucrose gradient centrifugation. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Subcellular fractionation of liver organelles from phenobarbital-treated rats by counter-current partition and sucrose gradient centrifugation.

Authors: Morris, WB  Smith, GD  Peters, TJ 
Citation: Morris WB, etal., Biochem Pharmacol. 1986 Jul 1;35(13):2187-91.
RGD ID: 1624347
Pubmed: PMID:3015147   (View Abstract at PubMed)

Counter-current partition, sucrose density gradient centrifugation and enzymic analysis were used to explore the changes in rat liver organelles induced by phenobarbital. There was a small increase in partition coefficient and marker enzyme activity of the endoplasmic reticulum. The modal density of the marker enzyme showed a significant decrease reflecting the proliferation of the smooth endoplasmic reticulum. The mitochondria showed a bimodal distribution with a small reduction of marker enzyme activity. In contrast, lysosomes and peroxisomes were relatively unaffected by phenobarbital treatment. Phenobarbital caused a small but statistically insignificant increase in gamma-glutamyl transferase activity: density gradient centrifugation studies indicated that the increased activity was predominantly in the biliary canalicular elements. In contrast, cytosolic gamma-glutamyl hydrolase activity was strikingly reduced by phenobarbital treatment.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GghRatresponse to xenobiotic stimulus  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ggh  (gamma-glutamyl hydrolase)


Additional Information