RGD Reference Report - Reduced adipsin mRNA and circulating adipsin protein are modulated by adrenal steroids in obese Zucker rats. - Rat Genome Database

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Reduced adipsin mRNA and circulating adipsin protein are modulated by adrenal steroids in obese Zucker rats.

Authors: Johnson, PR  Spiegelman, B  Rosen, B  Turkenkopf, I  Ree, H  Greenwood, MR 
Citation: Johnson PR, etal., Am J Physiol. 1990 Jul;259(1 Pt 2):R184-8.
RGD ID: 1624327
Pubmed: PMID:2197880   (View Abstract at PubMed)

We investigated expression of the adipose-specific serine protease adipsin in genetically obese Zucker rats and whether adrenalectomy modifies expression. Adipsin mRNA levels were determined by slot blot and Northern blot analysis of total RNA samples extracted from epididymal adipose tissue and isolated retroperitoneal adipocytes of obese (fa/fa) and homozygous lean (Fa/Fa) Zucker rats. Both 30-day-old and 4-mo-old animals were analyzed in experiment 1. In experiment 2, 10-wk-old obese and lean rats were either bilaterally adrenalectomized or sham operated, and adipsin mRNA levels were determined on tissue and cell samples 2 wk postsurgery. In both experiments, serum adipsin protein was determined by Western blot analysis and plasma insulin by radioimmunoassay. The data show that both adipsin mRNA and adipsin protein are reduced in obese compared with lean rats and that adrenalectomy restores these values toward normal in obese rats. The data thus suggest that adrenal steroids are involved in modulating adipsin expression in obese Zucker rats and that insulin may be an intermediary factor in such modulation.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CFDHumanobesity  ISOCfd (Rattus norvegicus)mRNA more ...RGD 
CfdRatobesity  IEP mRNA more ...RGD 
CfdMouseobesity  ISOCfd (Rattus norvegicus)mRNA more ...RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CfdRatextracellular space  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cfd  (complement factor D)

Genes (Mus musculus)
Cfd  (complement factor D)

Genes (Homo sapiens)
CFD  (complement factor D)


Additional Information