RGD Reference Report - A monoclonal antibody to rat liver arylsulfatase C and its application in immunohistochemistry. - Rat Genome Database

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A monoclonal antibody to rat liver arylsulfatase C and its application in immunohistochemistry.

Authors: Kawano, J  Kotani, T  Umeki, K  Oinuma, T  Ohtaki, S  Aikawa, E 
Citation: Kawano J, etal., J Histochem Cytochem. 1989 May;37(5):683-90.
RGD ID: 1601401
Pubmed: PMID:2703703   (View Abstract at PubMed)

We purified arylsulfatase C from rat liver microsomes and prepared a monoclonal antibody (P42C2) to the purified enzyme. By SDS-PAGE and immunoblotting analysis using P42C2, the molecular weight of the purified enzyme and of the enzyme in liver and kidney microsomes were estimated at 62,000 daltons. P42C2 caused little inhibition of arylsulfatase C activity, and was bound only slightly to liver microsomes. Localization of arylsulfatase C was studied at the light and electron microscopic level by the indirect immunoperoxidase method using P42C2. In rat liver, arylsulfatase C was detected mainly in the hepatocytes, and less frequently in endothelial cells, Kupffer's cells, and Ito's cells. In rat kidney, strong staining was observed in the straight portions of the proximal tubules. The podocytes, interstitial cells, endothelial cells, and epithelial cells of Henle's thin limbs were stained faintly. By electron microscopy, arylsulfatase C was found localized on the membranes of the endoplasmic reticulum and nuclear envelopes in these cells. These immunohistochemical findings agree with the localization demonstrated by an enzyme-histochemical method which we had previously developed.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
StsRatendoplasmic reticulum membrane  IDA  RGD 
StsRatnuclear envelope  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Sts  (steroid sulfatase)


Additional Information