RGD Reference Report - Expression of tropism-related genes in regenerating skeletal muscle of rats treated with cyclosporin-A. - Rat Genome Database

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Expression of tropism-related genes in regenerating skeletal muscle of rats treated with cyclosporin-A.

Authors: Miyabara, EH  Aoki, MS  Soares, AG  Moriscot, AS 
Citation: Miyabara EH, etal., Cell Tissue Res. 2005 Mar;319(3):479-89. Epub 2005 Jan 27.
RGD ID: 1600773
Pubmed: PMID:15726428   (View Abstract at PubMed)
DOI: DOI:10.1007/s00441-004-1027-y   (Journal Full-text)

This work was undertaken to provide further insights into the expression of tropism-related genes in regenerating skeletal muscle of adult rats treated with cyclosporin-A (CsA), a calcineurin inhibitor. Rats were treated with CsA for 5 days and, on the 6th day, were submitted to cryolesion of the soleus muscles. CsA treatment continued for 1, 10, and 21 days after cryolesion. Muscles were removed, frozen, and stored in liquid nitrogen. Body and muscle weights, histological sections stained with toluidine blue, and gene expression of the regeneration molecular markers, viz., desmin and neonatal myosin heavy chain, were assessed to confirm that cryolesion and CsA treatment were effective during the allowed regeneration time. Quantitative reverse transcription/polymerase chain reaction demonstrated that myostatin gene expression was not altered by either cryolesion or CsA treatment combined with cryolesion. Calpain-3 gene expression decreased at 1 day after cryolesion and also following CsA treatment combined with cryolesion. However, calpain-3 gene expression was strongly up-regulated (approximately five-fold) 10 days after cryolesion and returned to control levels at day 21. CsA treatment blocked calpain-3 gene expression rise induced by 10 days of cryolesion. Atrogin-1 gene expression was decreased at 1 day after cryolesion and following cryolesion combined with CsA treatment, returning to control levels at day 10. These results suggest that (1) calpain-3 has a differential role in the early and late stages of regeneration in a calcineurin-dependent manner, and (2) atrogin-1 is involved in the early stages of regeneration independently of calcineurin.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to xenobiotic stimulus  IEP 1600773 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Capn3  (calpain 3)


Additional Information