RGD Reference Report - Collaboration of PSD-Zip70 with its binding partner, SPAR, in dendritic spine maturity. - Rat Genome Database

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Collaboration of PSD-Zip70 with its binding partner, SPAR, in dendritic spine maturity.

Authors: Maruoka, H  Konno, D  Hori, K  Sobue, K 
Citation: Maruoka H, etal., J Neurosci. 2005 Feb 9;25(6):1421-30.
RGD ID: 1600102
Pubmed: PMID:15703396   (View Abstract at PubMed)
PMCID: PMC6726009   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.3920-04.2005   (Journal Full-text)

Recent studies have reported on the molecular mechanisms underlying dendritic spine (spine) dynamics. Because most of these studies investigated spine dynamics by overexpressing constitutively active or dominant-negative PSD (postsynaptic density) proteins in cultured mature neurons, the results represent the enlargement of mature spines or their return to an immature state. Here, we developed the technique of in utero electroporation to investigate spine dynamics. Using this technique, we demonstrated the suppression of spine maturation by the C-terminal variants of PSD-Zip70 in vitro and in vivo. Transient overexpression of the C terminus of PSD-Zip70 and knock-down of PSD-Zip70 also displayed the destabilization of mature spines. We further found the PSD-Zip70 and SPAR (spine-associated RapGAP) interaction via the short C-terminal region of PSD-Zip70 and the GK-binding domain of SPAR. In association with immature spines induced by overexpression of the PSD-Zip70 C terminus or knock-down of PSD-Zip70, SPAR lost its spine localization. Overexpression of the GK-binding domain of SPAR also induced to form immature spines without affecting the localization of PSD-Zip70 in the small heads of filopodial spines. Our results suggest that PSD-Zip70 in collaboration with SPAR is critically involved in spine maturity, especially in the mature spine formation and the maintenance of spine maturity.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Lzts1Ratregulation of dendrite morphogenesis  IMP  RGD 
Sipa1l1Ratregulation of dendrite morphogenesis  IMP  RGD 
Lzts1Ratregulation of synaptic plasticity  IMP  RGD 
Sipa1l1Ratregulation of synaptic plasticity  IMP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Lzts1Ratdendritic spine  IDA  RGD 
Sipa1l1Ratdendritic spine  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Lzts1Ratprotein binding  IPISipa1l1 (Rattus norvegicus) RGD 
Sipa1l1Ratprotein binding  IPILzts1 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lzts1  (leucine zipper tumor suppressor 1)
Sipa1l1  (signal-induced proliferation-associated 1 like 1)


Additional Information