RGD Reference Report - Interleukin-12 secreted by mature dendritic cells mediates activation of NK cell function. - Rat Genome Database

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Interleukin-12 secreted by mature dendritic cells mediates activation of NK cell function.

Authors: Alli, RS  Khar, A 
Citation: Alli RS and Khar A, FEBS Lett. 2004 Feb 13;559(1-3):71-6.
RGD ID: 1600048
Pubmed: PMID:14960310   (View Abstract at PubMed)
DOI: DOI:10.1016/S0014-5793(04)00026-2   (Journal Full-text)

Dendritic cells (DCs) are known to modulate immune response by activating effector cells of both the innate and the adaptive immune system. In the present study, we demonstrate that co-culture of DCs with paraformaldehyde-fixed tumor cells augments the secretion of interleukin (IL)-12 by DCs and these activated DCs upon co-culture with naive NK cells enhance the cytolytic activity of NK cells against NK-sensitive target YAC-1. Similarly, DCs isolated from tumor-bearing animals also activated NK cells in vitro. For efficient activation of NK cells, the ratio of activated DCs to NK cells is crucial. Addition of anti-IL-12 antibody to the culture system completely abolished activation of NK cells by DCs, suggesting that IL-12 secreted by DCs is an essential factor in NK cell activation. Adoptive transfer of DCs isolated from tumor-bearing animals into normal rats also induced activation of NK cells in normal animals.




Biological Process

  
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Original Reference(s)
Il12bRatnatural killer cell activation involved in immune response  IMP  RGD 


Genes (Rattus norvegicus)
Il12b  (interleukin 12B)