RGD Reference Report - Sodium retention in rats with liver cirrhosis is associated with increased renal abundance of NaCl cotransporter (NCC). - Rat Genome Database

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Sodium retention in rats with liver cirrhosis is associated with increased renal abundance of NaCl cotransporter (NCC).

Authors: Yu, Z  Serra, A  Sauter, D  Loffing, J  Ackermann, D  Frey, FJ  Frey, BM  Vogt, B 
Citation: Yu Z, etal., Nephrol Dial Transplant. 2005 Sep;20(9):1833-41. Epub 2005 Jun 14.
RGD ID: 1580587
Pubmed: PMID:15956070   (View Abstract at PubMed)
DOI: DOI:10.1093/ndt/gfh916   (Journal Full-text)

BACKGROUND: Liver cirrhosis is associated with enhanced renal tubular sodium retention, the mechanism of which is still debated. We hypothesized that liver cirrhosis is associated with increased expression of renal epithelial sodium transporter(s). METHODS: Liver cirrhosis was induced by bile duct ligation (BDL) in rats. Steady state mRNA of ENaC subunits alpha, beta, gamma serum and glucocorticoid inducible kinase (Sgk1) were measured by TaqMan PCR in kidney homogenates at week 1, 2, 3 and 4 after BDL. Renal protein content of ENaC subunits, ubiquitin-protein-ligase Nedd4-2 and NaCl cotransporter (NCC) were assessed by western blot. Subcellular localization of ENaC subunits and NCC were analysed by immunohistochemistry. RESULTS: Steady state mRNA of ENaC alpha, beta and gamma were unchanged during the 4 weeks investigated, while ENaC protein decreased most prominently at week 2 (control vs BDL; alpha, -46%; beta, -81%; and gamma, -63%; n = 6). Subcellular localization of ENaC subunits was not altered at week 2. Sgk1 mRNA did not change, whereas Nedd4-2 protein was reduced by >50% 2-4 weeks after BDL. NCC protein significantly increased at week 1 (control vs BDL: +66%, n = 6, P<0.05) and decreased at week 3 (control vs BDL: -85%, n = 6, P<0.0005). CONCLUSIONS: Enhanced abundance of NCC was observed in the initial stage after BDL, followed by a marked decrease. ENaC transcription, translation or cell surface abundance was not increased after BDL.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SLC12A3HumanExperimental Liver Cirrhosis  ISOSlc12a3 (Rattus norvegicus)protein:altered expression:kidney (rat)RGD 
Slc12a3RatExperimental Liver Cirrhosis  IEP protein:altered expression:kidney (rat)RGD 
Slc12a3MouseExperimental Liver Cirrhosis  ISOSlc12a3 (Rattus norvegicus)protein:altered expression:kidney (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc12a3  (solute carrier family 12 member 3)

Genes (Mus musculus)
Slc12a3  (solute carrier family 12, member 3)

Genes (Homo sapiens)
SLC12A3  (solute carrier family 12 member 3)


Additional Information