RGD Reference Report - Rat histamine N-methyltransferase. Quantification, tissue distribution, purification, and immunologic properties. - Rat Genome Database

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Rat histamine N-methyltransferase. Quantification, tissue distribution, purification, and immunologic properties.

Authors: Bowsher, R R  Verburg, K M  Henry, D P 
Citation: Bowsher RR, etal., J Biol Chem. 1983 Oct 25;258(20):12215-20.
RGD ID: 152985536
Pubmed: PMID:6415051   (View Abstract at PubMed)

Histamine N-methyltransferase is a major enzyme for inactivating histamine in mammalian tissues. Development of a sensitive and specific assay for histamine N-methyltransferase has permitted quantification of this activity in 25 rat tissues. Additionally, renal histamine N-methyltransferase was purified to electrophoretic homogeneity. The pivotal step in the purification scheme was an affinity column procedure which involved the specific elution of histamine N-methyltransferase from DEAE-Sephacel by 1 mM histamine. The purified enzyme has a molecular weight of 33,400 and a narrow pH dependency with an optimum at 8.0-8.25. Specific antibody produced to renal histamine N-methyltransferase also immunoprecipitated the brain enzyme completely, therefore indicating neuronal and non-neuronal histamine N-methyltransferase share common antigenic determinants.



Objects referenced in this article
Gene Hnmt histamine N-methyltransferase Rattus norvegicus

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