RGD Reference Report - Surfactant protein B propeptide contains a saposin-like protein domain with antimicrobial activity at low pH. - Rat Genome Database

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Surfactant protein B propeptide contains a saposin-like protein domain with antimicrobial activity at low pH.

Authors: Yang, Li  Johansson, Jan  Ridsdale, Ross  Willander, Hanna  Fitzen, Michael  Akinbi, Henry T  Weaver, Timothy E 
Citation: Yang L, etal., J Immunol. 2010 Jan 15;184(2):975-83. doi: 10.4049/jimmunol.0900650. Epub 2009 Dec 9.
RGD ID: 151667448
Pubmed: PMID:20007532   (View Abstract at PubMed)
PMCID: PMC2803329   (View Article at PubMed Central)
DOI: DOI:10.4049/jimmunol.0900650   (Journal Full-text)

Surfactant protein B (SP-B) proprotein contains three saposin-like protein (SAPLIP) domains: a SAPLIP domain corresponding to the mature SP-B peptide is essential for lung function and postnatal survival; the function of SAPLIP domains in the N-terminal (SP-BN) and C-terminal regions of the proprotein is not known. In the current study, SP-BN was detected in the supernatant of mouse bronchoalveolar lavage fluid (BALF) and in nonciliated bronchiolar cells, alveolar type II epithelial cells, and alveolar macrophages. rSP-BN indirectly promoted the uptake of bacteria by macrophage cell lines and directly killed bacteria at acidic pH, consistent with a lysosomal, antimicrobial function. Native SP-BN isolated from BALF also killed bacteria but only at acidic pH; the bactericidal activity of BALF at acidic pH was completely blocked by SP-BN Ab. Transgenic mice overexpressing SP-BN and mature SP-B peptide had significantly decreased bacterial burden and increased survival following intranasal inoculation with bacteria. These findings support the hypothesis that SP-BN contributes to innate host defense of the lung by supplementing the nonoxidant antimicrobial defenses of alveolar macrophages.




  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
SFTPBHumanPseudomonas Infections amelioratesIDA  RGD 
SftpbRatPseudomonas Infections amelioratesISORGD:737197 RGD 
SftpbMousePseudomonas Infections amelioratesISORGD:737197 RGD 
SFTPBHumanStaphylococcal Pneumonia amelioratesIDA  RGD 
SftpbRatStaphylococcal Pneumonia amelioratesISORGD:737197 RGD 
SftpbMouseStaphylococcal Pneumonia amelioratesISORGD:737197 RGD 


Genes (Rattus norvegicus)
Sftpb  (surfactant protein B)

Genes (Mus musculus)
Sftpb  (surfactant associated protein B)

Genes (Homo sapiens)
SFTPB  (surfactant protein B)