RGD Reference Report - Methylation of promoter and expression silencing of GPX3 gene in hepatocellular carcinoma tissue. - Rat Genome Database

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Methylation of promoter and expression silencing of GPX3 gene in hepatocellular carcinoma tissue.

Authors: Cao, Shengli  Yan, Bing  Lu, Yantao  Zhang, Gong  Li, Jie  Zhai, Wenlong  Guo, Wenzhi  Zhang, Shuijun 
Citation: Cao S, etal., Clin Res Hepatol Gastroenterol. 2015 Apr;39(2):198-204. doi: 10.1016/j.clinre.2014.09.003. Epub 2014 Oct 13.
RGD ID: 151665509
Pubmed: PMID:25445749   (View Abstract at PubMed)
DOI: DOI:10.1016/j.clinre.2014.09.003   (Journal Full-text)


BACKGROUND/AIMS: Accumulating evidence has identified transcriptional silencing by aberrant methylation of CpG islands as a potential mechanism for the inactivation of tumor suppressor genes. The role of aberrant methylation of the GPX3 promoter in hepatocellular carcinoma (HCC) is not yet clear. We investigated the association of the status of GPX3 promoter methylation and GPX3 protein expression with the clinicopathological progression of HCC.
METHODOLOGY: Sixty HCC tumor and matched non-cancerous tissues were included in this study, and methylation was examined using MSP. GPX3 mRNA and protein levels were evaluated using RT-PCR and western blot analysis, respectively. Clinicopathological data were compiled for correlation analyses.
RESULTS: Among the 60 HCC cases, 76.7% (46/60) showed at elevated DNA methylation and displayed significantly lower levels of GPX3 mRNA and protein expression. Low or undetectable GPX3 protein expression was observed in 10 of 60 tumors. GPX3 promoter methylation was detected in 46 of 60 (76.7%) tumors, while no GPX3 gene promoter methylation was observed in the matched non-cancerous specimens. There was a negative correlation between promoter methylation and GPX3 mRNA levels (P<0.05). Analysis of clinicopathological data revealed that both mRNA and protein were significantly associated with portal tumor thrombosis, metastasis and differentiation. In additional, GPX3 methylation showed a relationship with portal tumor thrombosis, metastasis and differentiation.
CONCLUSIONS: Our results suggest that promoter methylation may be a mechanism for inactivation of GPX3, possibly leading to subsequent carcinogenesis and progression of HCC.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GPX3Humanhepatocellular carcinoma disease_progressionIDA DNA:hypermethylationRGD 
Gpx3Rathepatocellular carcinoma disease_progressionISOGPX3 (Homo sapiens)DNA:hypermethylationRGD 
Gpx3Mousehepatocellular carcinoma disease_progressionISOGPX3 (Homo sapiens)DNA:hypermethylationRGD 

Objects Annotated

Genes (Rattus norvegicus)
Gpx3  (glutathione peroxidase 3)

Genes (Mus musculus)
Gpx3  (glutathione peroxidase 3)

Genes (Homo sapiens)
GPX3  (glutathione peroxidase 3)


Additional Information